Legionella Pneumophila Antibody (2F10) [Alexa Fluor® 647]

• Reactivity: Ba
• Applications: ELISA
• Clone: 2F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

Legionella Pneumophila Antibody (2F10) [Alexa Fluor® 647] Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with membrane fraction of Legionella pneumophila, strain Philadelphia 1.
• Specificity: Reacts with LPS membrane of Legionella pneumophila. No cross-reactivity with Legionella pneumophila serogroups 2, 6, 7, 10 and 11, Legionella bozemanii, Legionella lonbeachae, Legionella micdadei, Corynebacterium diphtheria, Pseudomonas fluorescens, Pseudomonas cepacia, Pseudomonas aerugenose, Bordetella pertusis, Leptospira interrogens (Australia, Pomona, Icterogemorrhagia), Toxoplasma gondii, Hemophilus influenza (type B), Hemophilus ducreyi, Brucella abortus, Bacillus anthracis, Klebsiella pneumoniae, Listeria monocytogenes, Mycobacterium tuberculosis, Neisseria meningitides, Pasteurella multocida, Proteus vulgaris, Shigella sonnei, Staphylococcus faecalis, Streptococcus puogenes, Streptococcus pneumoniae, Yersinia pseudotuberculosis, Yersinia pestis EV76, Salmonella typhi, Escherichia coli K88 and Francisella tularensis 15. Cross-reactivity with Legionella pneumophila serogroups 3 and 4 has not been tested.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Bacteria

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Legionella Pneumophila Antibody (2F10) [Alexa Fluor® 647]

• L. pneumophila
• Legionellosis

Background

Legionella pneumophila is a flagellated gram negative bacterium found primarily in warm water environments. Legionella pneumophila causes a type of pneumonia called Legionnaire disease and a milder condition called Pontiac fever. Infection is acquired through inhalation of the microorganism in water mists (e.g. from air conditioning, cooling towers, whirlpool spas and showers mists). A number of risk factors for acquiring Legionnaire disease have been identified, including age, smoking, chronic lung disease, cancer, and immunosuppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.