Legionella Pneumophila Antibody (2F10) [Janelia Fluor® 669]

• Reactivity: Ba
• Applications: ELISA
• Clone: 2F10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Janelia Fluor 669

Legionella Pneumophila Antibody (2F10) [Janelia Fluor® 669] Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with membrane fraction of Legionella pneumophila, strain Philadelphia 1.
• Specificity: Reacts with LPS membrane of Legionella pneumophila. No cross-reactivity with Legionella pneumophila serogroups 2, 6, 7, 10 and 11, Legionella bozemanii, Legionella lonbeachae, Legionella micdadei, Corynebacterium diphtheria, Pseudomonas fluorescens, Pseudomonas cepacia, Pseudomonas aerugenose, Bordetella pertusis, Leptospira interrogens (Australia, Pomona, Icterogemorrhagia), Toxoplasma gondii, Hemophilus influenza (type B), Hemophilus ducreyi, Brucella abortus, Bacillus anthracis, Klebsiella pneumoniae, Listeria monocytogenes, Mycobacterium tuberculosis, Neisseria meningitides, Pasteurella multocida, Proteus vulgaris, Shigella sonnei, Staphylococcus faecalis, Streptococcus puogenes, Streptococcus pneumoniae, Yersinia pseudotuberculosis, Yersinia pestis EV76, Salmonella typhi, Escherichia coli K88 and Francisella tularensis 15. Cross-reactivity with Legionella pneumophila serogroups 3 and 4 has not been tested.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Legionella Pneumophila Antibody (2F10) [Janelia Fluor® 669]

• L. pneumophila
• Legionellosis

Background

Legionella pneumophila is a flagellated gram negative bacterium found primarily in warm water environments. Legionella pneumophila causes a type of pneumonia called Legionnaire disease and a milder condition called Pontiac fever. Infection is acquired through inhalation of the microorganism in water mists (e.g. from air conditioning, cooling towers, whirlpool spas and showers mists). A number of risk factors for acquiring Legionnaire disease have been identified, including age, smoking, chronic lung disease, cancer, and immunosuppression.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.