Goat anti-Human Lambda Light Chain Secondary Antibody

Images

 
Western Blot: Goat anti-Human Lambda Light Chain Secondary Antibody [Unconjugated] [NBP1-72788] - Lane 1: Human lambda (lambda chain). Lane 2: None. Load: 100 ng per lane. Primary antibody: Human lambda (lambda chain) ...read more
Immunocytochemistry/ Immunofluorescence: Goat anti-Human Lambda Light Chain Secondary Antibody [Unconjugated] [NBP1-72788] - Human multiple myeloma cell lines 8226, LP-1, H929. 1. Cells immersion-fixed in 4% PFA for 10 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Goat anti-Human Lambda Light Chain Secondary Antibody Summary

Immunogen
Goat anti-Human Lambda Light Chain Secondary Antibody was produced by repeated immunization with Human lambda light chain molecule in goat.
Specificity
Specificity was confirmed by ELISA.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
IGLC1
Purity
Multi-step

Applications/Dilutions

Dilutions
  • ELISA 1:4000 - 1:20000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:500 - 1:2000
Application Notes
This secondary antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-72788 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
Preservative
0.1% Sodium Azide
Purity
Multi-step

Notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. Solid phase adsorption(s) against a panel of Human IgG Kappa, IgM Kappa and IgA Kappa proteins was performed

Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Alternate Names for Goat anti-Human Lambda Light Chain Secondary Antibody

  • IGLC
  • immunoglobulin lambda constant group

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Lambda Light Chain Antibody (NBP1-72788) (0)

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Review for Lambda Light Chain Antibody (NBP1-72788) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-72788:
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Immunocytochemistry
(1)
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Images Ratings Applications Species Date Details
Immunocytochemistry Lambda Light Chain NBP1-72788
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5
reviewed by:
Verified Customer
Immunocytochemistry Human 10/11/2019
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Summary

ApplicationImmunocytochemistry
Sample Testedmultiple myeloma cell lines 8226
SpeciesHuman
Lot41513

Comments

Comments1. Cells immersion-fixed in 4%PFA 10 minutes, wash PBS X 3
2. Permeabilized 0.5% Triton X-100 5 minutes; wash PBS X 3
3. Block 10% donkey serum 1 hour
4. 1/5000 NBP1-72788 O/N @4oC; wash PBS X 3
5. 1/2000 donkey F(ab’)Cy3 anti-goat IgG (H+L) 1 hour @room temp (Jackson #705-166-147); wash PBS X 3
6. Olympus/BIOVIEW Cy3 filter 300ms exposure

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Lambda Light Chain Antibody (NBP1-72788) (0)

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
10/11/2019
Application: Immunocytochemistry
Species: Human

Bioinformatics

Gene Symbol IGLC1