Goat anti-Human Lambda Light Chain Secondary Antibody

Western Blot: Goat anti-Human Lambda Light Chain Secondary Antibody [Unconjugated] [NBP1-72788] - Lane 1: Human lambda (lambda chain). Lane 2: None. Load: 100 ng per lane. Primary antibody: Human lambda (lambda chain) Antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight800 Donkey anti-Goat at 1:20,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Other Band(s): None.

Immunocytochemistry/Immunofluorescence: Goat anti-Human Lambda Light Chain Secondary Antibody [Unconjugated] [NBP1-72788] - Human multiple myeloma cell lines 8226, LP-1, H929. 1. Cells immersion-fixed in 4% PFA for 10 minutes, washed 3x with PBS. Permeabilized with 0.5% Triton X-100 for 5 minutes, washed 3x with PBS. Blocked with 10% donkey serum 1 hour. NBP1-72788 antibody at 1:5000 O/N at 4C, washed 3x with PBS. Secondary antibody at 1:2000 donkey F(ab')Cy3 anti-goat IgG (H+L) 1 hour at room temp (Jackson #705-166-147); washed 3x with PBS. Imaging with Olympus/BIOVIEW Cy3 filter 300ms exposure. ICC/IF image submitted by a verified customer review.

Western Blot of Goat anti-Human lambda chain Antibody. Lane 1: Human lambda chain. Lane 2: None. Load: 100 ng per lane. Primary antibody: Human lambda chain Antibody at 1:1000 for overnight at 4C. Secondary antibody: DyLight800 Donkey anti-Goat at 1:20,000 for 30 min at RT. Block for 30 min at RT. Other Band(s): None.

Product Discontinued

Goat anti-Human Lambda Light Chain Secondary Antibody Summary

• Description: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Human IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum. Solid phase adsorption(s) against a panel of Human IgG Kappa, IgM Kappa and IgA Kappa proteins was performed
  
  Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Goat anti-Human Lambda Light Chain Secondary Antibody was produced by repeated immunization with Human lambda light chain molecule in goat.
• Specificity: Specificity was confirmed by ELISA.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IGLC1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:4000 - 1:20000
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:1000 - 1:5000
  • Western Blot 1:500 - 1:2000
• Application Notes: This product has been tested by ELISA and western blot and is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
• Preservative: 0.1% Sodium Azide
• Purity: Multi-step

Alternate Names for Goat anti-Human Lambda Light Chain Secondary Antibody

• IGLC
• immunoglobulin lambda constant group

Background

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Review for Lambda Light Chain Antibody (NBP1-72788) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Natalie Erdmann ICC Human 10/11/2019

Summary

• Application: Immunocytochemistry
• Sample Tested: multiple myeloma cell lines 8226
• Species: Human
• Lot: 41513

Comments

• Comments: 1. Cells immersion-fixed in 4%PFA 10 minutes, wash PBS X 3
  2. Permeabilized 0.5% Triton X-100 5 minutes; wash PBS X 3
  3. Block 10% donkey serum 1 hour
  4. 1/5000 NBP1-72788 O/N @4oC; wash PBS X 3
  5. 1/2000 donkey F(ab’)Cy3 anti-goat IgG (H+L) 1 hour @room temp (Jackson #705-166-147); wash PBS X 3
  6. Olympus/BIOVIEW Cy3 filter 300ms exposure

Bioinformatics

• Gene Symbol: IGLC1