Lambda Light Chain Antibody (ICO-106)

Western Blot: Lambda Light Chain Antibody (HP6054) [NBP2-22385] - Analysis of Lambda in human intestine using Lambda HP6054 antibody at 2 ug/ml, HRP secondary and ECL substrate.

Immunocytochemistry/Immunofluorescence: Lambda Light Chain Antibody (HP6054) [NBP2-22385] - Confocal Immunofluorescent analysis of Ramos cells using AF488-labeled isotype control Monoclonal Antibody (IgG2a) (Green) DAPI was used to stain the cell nuclei ( blue)/ (Negative control

Immunohistochemistry-Paraffin: Lambda Light Chain Antibody (HP6054) [NBP2-22385] - Formalin-paraffin human tonsil stained with Lambda Light Chain Ab (LcN-2). Note cell membrane & cytoplasmic staining.

Immunocytochemistry/Immunofluorescence: Lambda Light Chain Antibody (HP6054) [NBP2-22385] - Confocal immunofluorescent analysis of Ramos cellsusing Af488-labeled Lambda Light Chain Monoclonal Antibody (LcN-2) (Green). DAPI was used to stain the cell nuclei (blue).

Product Discontinued

Lambda Light Chain Antibody (ICO-106) Summary

• Immunogen: Purified human IgG myeloma proteins covalently coupled to polyaminostyren microbeads were used as the immunogen for the HP6054 antibody.
• Localization: Cell Surface, Cytoplasmic and Secreted
• Marker: B-Cell Marker
• Specificity: This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: IGLC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1-5ug/ml for coating
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
  • Western Blot 0.5-1ug/ml
• Application Notes: Human Chromosome Location: 22q11.22
  Immunohistochemistry-Frozen application reported in literature. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. The Lambda HP6054 antibody is useful for the identification of immunoglobulins in leukemias, plasmacytomas and some non-Hodgkins lymphomas. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 22.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A purified

Alternate Names for Lambda Light Chain Antibody (ICO-106)

• IGLC
• immunoglobulin lambda constant group

Background

All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins). This antibody stains only lambda light chain containing cells in human lymphoid tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IGLC1
• Uniprot:
  • AAH94465.1(Rat)