KSHV ORF26 Antibody (2F6B8)

Western Blot: KSHV ORF26 Antibody (2F6B8) [NBP1-47357] - Analysis using KSHV ORF26 mouse mAb against TPA induced BCBL-1 cell lysate.

KSHV lacking SOX produces fewer infectious virions. (A) Western blot of whole cell lysates from iSLK cells containing a KSHV mutant that does not express SOX (SOX stop) or a mutant rescue version of KSHV where SOX expression is restored (SOX stop MR). Lysates were harvested 72 hours post lytic reactivation and blotted with an anti-SOX antibody. Vinculin serves as a loading control. (B, C) RT-qPCR was used to measure the RNA levels of several host (B) or viral (C) transcripts at 48 hours post-reactivation in iSLK cell lines containing the indicated WT or mutant KSHV. Transcript levels were normalized to 18S and to the WT iSLK unreactivated population for (B) for the WT iSLK reactivated population (C). A minimum of three biological replicates are shown. The peak timing of each of the viral genes’ expression is indicated below their name (57). (D) Western blot of lysates from the indicated iSLK cell lines that were either unreactivated or reactivated for 48 hours showing expression of late viral proteins ORF26 and K8.1. Vinculin is used as a loading control. (E) RT-qPCR was used as in (C) to measure the levels of the indicated viral transcripts, but in the presence of 500 mM of the viral DNA replication inhibitor phosphonoacetic acid (PAA). *P ≤ 0.05, one-way ANOVA followed by Dunnett's multiple comparisons test versus WT iSLK. (F) Infectious virion production was measured by flow cytometry on 1 × 106 293T cells infected with 1 ml of supernatant from 1 × 106 reactivated iSLKs. Bars represent the percentage of naïve 293T cells that became GFP positive after 24 hours. Data are from a minimum of four independent biological replicates. ****P ≤ 0.001, one-sample t test versus hypothetical value of 1. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36537232), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: V-Vi
• Applications: WB, ELISA
• Clone: 2F6B8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

KSHV ORF26 Antibody (2F6B8) Summary

• Immunogen: Purified recombinant fragment of KSHV ORF26 expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ORF26
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1:500-1:2000

Reactivity Notes

Kaposi's sarcoma herpes virus

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Ascites

Alternate Names for KSHV ORF26 Antibody (2F6B8)

• HHV8
• Human Herpes Virus 8
• Kaposi's sarcoma herpes virus
• KSHV
• ORF45

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for KSHV ORF26 Antibody (NBP1-47357)(10)

Publications using NBP1-47357

• Harrington E, Murphy J, Harper K et al. EMG1 methyltransferase activity affects ribosome occupancy at KSHV uORFs. Cell reports 2025-04-10 [PMID: 40215162]
• McCollum C, Didychuk A, Liu D et al. The viral packaging motor potentiates late gene expression in Kaposi�s sarcoma-associated herpesvirus bioRxiv 2023-02-01 [PMID: 37068108] (Western Blot, Virus)
• Hartenian E, Mendez AS, Didychuk AL et al. DNA processing by the Kaposi's sarcoma-associated herpesvirus alkaline exonuclease SOX contributes to viral gene expression and infectious virion production Nucleic Acids Research 2023-01-11 [PMID: 36537232] (Western Blot, Human)
• Fiches GN, Wu Z, Zhou D et al. Polyamine biosynthesis and eIF5A hypusination are modulated by the DNA tumor virus KSHV and promote KSHV viral infection PLOS Pathogens 2022-04-29 [PMID: 35486659] (Western Blot, Human)
• Golas G, Alonso JD, Toth Z Characterization of de novo lytic infection of dermal lymphatic microvascular endothelial cells by Kaposi's sarcoma-associated herpesvirus Virology 2019-07-31 [PMID: 31394409] (WB, Human)
• Haque M, Kousoulas KG The KSHV ORF34 protein Interacts and Stabilizes HIF-2 alpha via Binding to the HIF-2 alpha bHLH and PAS Domains J. Virol. 2019-06-12 [PMID: 31189709]
• Papp B, Motlagh N, Smindak RJ et al. Genome-wide identification of direct RTA targets reveals key host factors for KSHV lytic reactivation J. Virol. 2018-12-12 [PMID: 30541837] (WB, Human)
• Golas G, Jang S, Naik N, et al. Comparative analysis of the viral interferon regulatory factors of KSHV for their requisite for virus production and inhibition of the type I interferon pathway Virology 2020-02-15 [PMID: 32056714]

FAQs for KSHV ORF26 Antibody (NBP1-47357). (Showing 1 - 1 of 1 FAQs).

1. I need to do Viral IL-6 (HHV8) ELISA. I was wondering if you have the kit if not would it be possible to make it for us? I would appreciate if you inform me with all the details.
   • We do not have an HHV8 kit, but we have two HHV8 antibodies that may be used for an ELISA: NBP1-30176 and NBP1-47357.

Bioinformatics

• Gene Symbol: ORF26
• Entrez:
  • 4961509(Human)