Japanese Encephalitis virus NS1 Antibody (995047) [Unconjugated]

Recombinant Viral Japanese Encephalitis virus NS1 protein was serially diluted 2-fold and captured by Mouse Anti-Viral Japanese Encephalitis virus NS1 Monoclonal Antibody (Catalog # MAB100062) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Viral Japanese Encephalitis virus NS1 Monoclonal Antibody (Catalog # MAB100061) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: V
• Applications: ELISA
• Clone: 995047
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Japanese Encephalitis virus NS1 Antibody (995047) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived viral Japanese Encephalitis virus NS1 protein
  Thr798-Ala1146
  Accession # P32886
• Specificity: Detects viral Japanese Encephalitis virus NS1 protein in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Japanese Encephalitis virus NS1 Antibody (995047) [Unconjugated]

• Japanese Encephalitis virus NS1
• Japanese Encephalitis
• JE virus
• JEV
• JEVgp1
• JEV-NS1

Background

Japanese encephalitis virus (JEV) non-structural protein 1 (NS1) is produced by the Japanese encephalitis virus (JEV), a mosquito-borne flavivirus that is the leading cause of virus-induced encephalitis globally (1). Flavivirus NS1 is a multifunctional virulence factor. The glycosylated NS1 exists as a membrane-associated dimer after translocation into the endoplasmic reticulum lumen, where it is essential for viral genome replication (2). The secreted hexamer NS1 is involved in immune evasion and pathogenesis; it is identified as a potential diagnostic marker for early detection of the virus infections (2). Mature viral JEV NS1 contains 352 amino acids and shares high structural similarity to other flavivirus NS1 proteins, such as DENV and ZIKV.

1. Nain, M. et al. (2016) Rev. Med. Virol. 26:129.
2. Rastogi, M. et al. (2016) Virol. J. 13:131.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P32886()