![Flow Cytometry: Influenza M1 / HLA-A2 Complex Antibody (405H1.01) [DDX0270P-100] - M1 loading on HLA.A2 transfected EL4 cells:Flow cytometry with clone 405H1.01 Control: EL4 HLA-A2 cells pulsed with M1 peptide, no primary antibodyEL4 HLA-A2 cells pulsed with peptide M1 stained with 405H1 mAbMixture of EL4 & EL4 HLA-A2 cells pulsed with M1, stained with 405H1 mAb](http://images.novusbio.com/fullsize/Influenza-M1-HLA-A2-Complex-Antibody-405H1-01-Flow-Cytometry-DDX0270P-100-img0003.jpg "Flow Cytometry: Influenza M1 / HLA-A2 Complex Antibody (405H1.01) [DDX0270P-100] - M1 loading on HLA.A2 transfected EL4 cells:Flow cytometry with clone 405H1.01 Control: EL4 HLA-A2 cells pulsed with M1 peptide, no primary antibodyEL4 HLA-A2 cells pulsed with peptide M1 stained with 405H1 mAbMixture of EL4 & EL4 HLA-A2 cells pulsed with M1, stained with 405H1 mAb")
| Reactivity | V-ViSpecies Glossary |
| Applications | ELISA, Flow |
| Clone | 405H1.01 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 0.5 mg/ml |
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | DDX0270A488-100 | |||
| Alexa Fluor 647 | DDX0270A647-100 | |||
| Biotin | DDX0270B-100 | |||
| Immunogen | Influenza M1/HLA-A2 Complex was used as immunogen. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Ion exchange chromatography |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris-NaCl (pH 8.0) |
| Preservative | No Preservative |
| Concentration | 0.5 mg/ml |
| Purity | Ion exchange chromatography |
| Publication using DDX0270P-100 | Applications | Species |
|---|---|---|
| Wang X, Zhang H, Wang Y et al. DNA sensing via the cGAS/STING pathway activates the immunoproteasome and adaptive T-cell immunity The EMBO journal 2023-03-13 [PMID: 36912165] (FLOW, Human) Details: Dilution used in Flow 1:100 |
FLOW | Human |
Secondary Antibodies |
Isotype Controls |
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![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB](https://images.novusbio.com/images/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-HRP-Immunohistochemistry-Paraffin-NB7539-img0014.jpg "Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")