Influenza A nucleoprotein Antibody (7B4G10G8) - Azide and BSA Free

Western Blot: Influenza A nucleoprotein Antibody (7B4G10G8) [NBP3-23452] - NBP3-23452 at 1:1000. Lane A: Influenza A H1N1 nucleoprotein (30ng). Secondary Goat Anti-Mouse IgG H+L [DyLight 800] used at 1/15000 dilution. Developed using the Odyssey technique. Performed under reducing conditions.

• Reactivity: V-Vi
• Applications: WB, ELISA
• Clone: 7B4G10G8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

Influenza A nucleoprotein Antibody (7B4G10G8) - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: This product is a recombinant monoclonal antibody expressed from HEK293 cells.
• Source: HEK293
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:2000
  • Western Blot 1:1000-1:5000

Reactivity Notes

Has cross-reactivity in ELISA with H1N1 nucleoprotein, H7N9 (A/Anhui/1/2013) nucleoprotein, H7N9 (A/Shanghai/1/2013) nucleoprotein. Has no cross-reactivity in ELISA with insect cell lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.02% Tween80, pH7.0
• Preservative: 10% Trehalose
• Purity: Protein A purified

Alternate Names for Influenza A nucleoprotein Antibody (7B4G10G8) - Azide and BSA Free

• Influenza A virus NP
• NP
• NP-A
• Nucleocapsid protein
• Nucleoprotein

Background

The nucleoprotein (NP) of Influenza virus encapsulates the negative strand of the viral RNA and is essential for replicative transcription. It may also be involved in other essential functions throughout the virus life cycle. As well as binding ssRNA, NP is able to self associate to form large oligomeric complexes. NP is able to interact with a variety of other macromolecules of both viral and cellular origins. It binds the PB1 and PB2 subunits of the polymerase and the matrix protein M1. "NP has also been shown to interact with at least four cellular polypeptide families: nuclear import receptors of the importin class, filamentous (F) actin, the nuclear export receptor CRM1 and a DEAD box helicase BAT1/UAP56" (Portela et al 2002).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.