Influenza A Hemagglutinin H3 Antibody (InA227) - BSA Free

• Reactivity: V-Vi
• Applications: WB, ELISA, ICC/IF
• Clone: InA227
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Influenza A Hemagglutinin H3 Antibody (InA227) - BSA Free Summary

• Immunogen: Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with Influenza A/Shangdong/9/93 (strain H3N2).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:100-1:2000
• Application Notes: Influenza A H3 hemagglutinin immunodetection in direct or indirect ELISA. Detect Influenza A H3 hemagglutinin in Western Blotting. Use in ICC/IF was reported in scientific literature (PMID: 23749101).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Background

Influenza A belongs to the Orthomyxoviridae family, and is a negative sense single-stranded RNA virus which results in respiratory disease. Haemagglutinin is an antigenic glycoprotein which allows viral attachment and entry into the cell. Sixteen subtypes of haemagglutinin (H1-H16) have been described, of which H1, H2 and H3 infect humans. H5 and H7 normally result in avian disease, with some highly pathogenic H5N1 strains causing 100% mortality in poultry. H5N1 has in some cases mutated to infect humans, with 60% mortality. Haemagglutinin is one of the most medically relevant antigens on influenza as it is a target for antiviral drugs and antibodies. Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified avian influenza virus type A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Influenza A Hemagglutinin H3 Antibody (NB100-73161)(2)

Publications using NB100-73161

• Xu S, Zhou J, Liu Q et al. Evidences for the existence of intermolecular disulfide-bonded oligomers in the H3 hemagglutinins expressed in insect cells. Virus Genes. 2013-12-03 [PMID: 24297311] (WB, Virus)
• Xu S, Zhou J, Liu K et al. Mutations of two transmembrane cysteines of hemagglutinin (HA) from influenza A H3N2 virus affect HA thermal stability and fusion activity. Virus Genes 2013-06-08 [PMID: 23749101] (ICC/IF, WB, Virus)