Influenza A Hemagglutinin Antibody (1E6A7)

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Western Blot: Influenza A Hemagglutinin Antibody (1E6A7) [NBP1-75493] - analysis of (A) 5 ng and (B) 25 ng of recombinant HA1 with Hemagglutinin antibody at 1 ug/mL

Product Details

Summary
Reactivity ViSpecies Glossary
Applications WB, ELISA
Clone
1E6A7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Influenza A Hemagglutinin Antibody (1E6A7) Summary

Immunogen
A peptide corresponding to 13 amino acids in the middle of the Hemagglutinin protein.
Clonality
Monoclonal
Host
Mouse
Gene
HA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
Application Notes
Hemagglutinin antibody can be used for the detection of the Hemagglutinin protein from the H5N1 strain of avian influenza A in ELISA. It will detect 10 ng of free peptide at 1 μg/mL. Other applications are pending.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Influenza A Hemagglutinin Antibody (1E6A7)

  • HA epitope tag
  • HA1
  • HA2
  • Hemagglutinin

Background

Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA (1). Novel influenza virus strains caused by genetic drift and viral recombination emerge periodically to which humans have little or no immunity, resulting in devastating pandemics. Influenza A can exist in a variety of animals; however it is in birds that all subtypes can be found (2). These subtypes are classified based on the combination of the virus coat glycoproteins hemagglutinin (HA) and neuraminidase (NA) subtypes. During 1997, an H5N1 avian influenza virus was determined to be the cause of death in 6 of 18 infected patients in Hong Kong (3). The more recent virulent strain of H5N1 is now seen in Africa and Europe, as well as in southeast Asia. There is some evidence of human to human spread of this virus, but it is thought that the transmission efficiency was fairly low (4). HA interacts with cell surface proteins containing oligosaccharides with terminal sialyl residues. Virus isolated from a human infected with the H5N1 strain in 1997 could bind to oligosaccharides from human as well as avian sources, indicating its species-jumping ability (5). While efforts were made to use relatively conserved regions of the viral sequence as the antigen, the influenza virus genome has drifted somewhat from what was first reported. However, this antibody was able to recognize peptides derrived from viruses from Indonesian human patients infected in 2007.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional Influenza A Hemagglutinin Products

Influenza A Hemagglutinin NBP1-75493

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Bioinformatics

Gene Symbol HA
Uniprot