Mouse IgG2b Isotype Control (MB2) [HRP]

Product Discontinued

Mouse IgG2b Isotype Control (MB2) [HRP] Summary

• Description: This Mouse IgG2B immunoglobulin is useful as an isotype control for Mouse IgG2B antibody.
• Specificity: Typically, less than 1% cross reactivity against other mouse and human heavy or light chains isotypes was detected by ELISA.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:1000 - 1:5000
• Application Notes: This isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
  
  NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
• Preservative: 0.01% Gentamicin Sulfate
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent).

Notes

This product has been prepared from immunodeficient mouse ascites by protein A chromatography using specific conditions for subclass purification

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Alternate Names for Mouse IgG2b Isotype Control (MB2) [HRP]

• IgG2B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.