Mouse IgG2b Isotype Control (M2B)

SDS-Page: Mouse IgG2b Isotype Control (M2B) [NBP1-96969] - SDS-PAGE results of Mouse IgG2b isotype control (M2B).Lane 1: reduced Mouse IgG2b isotype control (M2B).Lane 2: opal prestained molecular weight ladder.Lane 3: non-reduced Mouse IgG2b isotype control (M2B).Load: 1ug.4-20% Lonza SDS-PAGE; Coomassie Stained; ChemiDoc Imaged.

• Reactivity: Mu
• Applications: WB, ELISA, Flow, ICC/IF, PAGE
• Clone: M2B
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Purified

Mouse IgG2b Isotype Control (M2B) Summary

• Description: This antibody has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Extensive cross-adsorption was performed to remove any unwanted subclasses. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgA, IgM. Confirmed by SDS-PAGE.
  
  Store vial at 4C prior to opening. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Specificity: Extensive cross-adsorption was performed to remove any unwanted subclasses. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgA, IgM
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • SDS-Page
  • Western Blot 1:100 - 1:2000
• Application Notes: This product has been tested by SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Protein A purified

Alternate Names for Mouse IgG2b Isotype Control (M2B)

• IgG2B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

FAQs for IgG2b Isotype Control (NBP1-96969). (Showing 1 - 1 of 1 FAQ).

1. Could you advise if I can use NBP1-96969 Ab as a control Ab for HIF1a for ChIP assay. I have a problem that normal rabbit IgG that usually are provided in the ChIP kits show higher binding compare to NB100-105 HIF1A Ab. Could you suggest if it is better to use Mouse IgG 2b Isotype control in this case or normal rabbit IgG should work as well? NBP1-96969 Ab are not validated for ChIP, please, let me know if there is something better? 
   • Yes, it would be reasonable to use a mouse IgG2b control for NB100-105 (HIF1 alpha) in ChIP assay. The control provided in the ChIP kit is recommended as a control for the histone antibodies (which are rabbit polyclonal). NBP1-96969 would be a good choice for control for the mouse monoclonal NB100-105. It has not yet been validated for ChIP, but it has been validated for use in IP, so I think it will be a good choice for control.