Mouse IgG2b Isotype Control (M2B)

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, FLISA, Flow, ICC/IF
Clone
M2B
Host
Mouse
Conjugate
Unconjugated

Order Details

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Mouse IgG2b Isotype Control (M2B) Summary

Description
This Mouse IgG2B immunoglobulin is useful as an isotype control for Mouse IgG2B antibody.
Specificity
Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Extensive cross-adsorbtion was performed to remove any unwanted subclasses. Typically less than 1% cross reactivity against other mouse and human heavy or light chains isotypes was detected by ELISA.
Isotype
IgG2b
Host
Mouse
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:1000 - 1:5000
  • Fluorophore-linked immunosorbent assay 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
Application Notes
Mouse IgG2b isotype control can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Stabilizer: None.
Preservative
0.01% Sodium Azide
Purity
Protein A purified

Alternate Names for Mouse IgG2b Isotype Control (M2B)

  • IgG2B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG2b Isotype Control (NBP1-96969). (Showing 1 - 1 of 1 FAQ).

  1. Could you, advice if I can use NBP1-96969 Ab as a control Ab for HIF1a for ChIP asay. I have a problem that normal rabbit IgG that usually are provided in the ChIP kits show higher binding compare to NB100-105 HIF1A Ab. Could you suggest if it is better to use Mouse IgG 2b Isotype control in this case or normal rabbit IgG should work as well? NBP1-96969 Ab are not validated for ChIP, please, let me know if there is something better?
    • Yes, it would be reasonable to use a mouse IgG2b control for NB100-105 (HIF1 alpha) in ChIP assay. The control provided in the ChIP kit is recommended as a control for the histone antibodies (which are rabbit polyclonal). NBP1-96969 would be a good choice for control for the mouse monoclonal NB100-105. It has not yet been validated for ChIP, but it has been validated for use in IP, so I think it will be a good choice for control.

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