Goat anti-Mouse IgG2b Secondary Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, DB, ELISA, IB, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Goat anti-Mouse IgG2b Secondary Antibody Summary

Immunogen
Pools of purified homogenous IgG2b isolated from pooled mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
The reactivity of the antiserum is directed to the subclass IgG2b. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immuno- electrophoresis and double radial immunodiffusion.
Clonality
Polyclonal
Host
Goat
Purity
Ion exchange chromatography

Applications/Dilutions

Dilutions
  • Dot Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunoblotting 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Western Blot
Application Notes
Indirect immunofluorescence

Reactivity Notes

This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. However this product has been passed over appropriate immuno- adsorbents to remove antibodies cross-reacting with Human immunoglobulins. This renders it specific for use in test systems containing material of Human origin (e.g. Human tissue/Mouse monoclonal antibody to a Human tissue constituent/anti Mouse Ig isotype-specific immunoconjugate. If the IgG fraction is to be used in the presence of material originating from another species, prior screening for cross-reactivity is essential an additional adsorption of the reagent may required to ensure intra-assay specificity.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.2)
Preservative
No Preservative
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.

Notes

The lyophilized IgG (7S) fraction is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Alternate Names for Goat anti-Mouse IgG2b Secondary Antibody

  • IgG2B

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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