Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody

Western Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Western Blot of Rabbit anti-Mouse IgG2a Heavy Chain Secondary antibody. Lane 1: Mouse IgG1. Lane 2: Mouse IgG2a. Lane 3: Mouse IgG2b. Lane 4: Mouse IgG3. Lane 5: Mouse IgM. Load: 5 ng per lane.Primary antibody: Mouse IgG2a antibody at 1 ug/mL for overnight at 4C.Secondary antibody: Gt-anti-Rabbit HRP at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 50 kDa and25 kDa, 50 kDa and 25 kDa for Mouse IgG2a. Other band(s): Weak reactivity for to Mouse IgG1.

Flow Cytometry: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Antibody [DyLight 405] [NBP1-72921]

Western Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Lane 1: Mouse IgG2a. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG2a Antibody Alkaline Phosphatase Conjugated at 1:1,000 o/n at 4C. Block: incubated with blocking buffer for 30 min at RT. Reaction visualized with alkaline phosphatase substrate for 30 seconds at RT. Predicted/Observed size: 55kDa for Mouse IgG2a.

Dot Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Mouse IgG2a Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4 Lane 2.5ng

Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Rabbit anti-Mouse IgG2a Antibody [DyLight 800] [NBP1-72915]

Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794] - Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Secondary Antibody [DyLight 488] [NBP1-72891] Image from the DyLight 488 version of this antibody.

Dot Blot: Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody [NBP1-72794]

Dot Blot of Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody. Tested with dilutions 10ng, 3.3ng, 1.1ng, 0.37ng, 0.12ng. Lane 1: Mouse IgG1

• Reactivity: Mu
• Applications: WB, DB, ELISA, Flow, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Rabbit anti-Mouse IgG2a Heavy Chain Secondary Antibody Summary

• Description: This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum and Mouse IgG2a. No arcs were detected against IgG1, IgG2b and IgG3
  
  Store vial at 4C prior to opening. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.
• Immunogen: Mouse IgG2a heavy chain
• Specificity: Specificity was confirmed by ELISA. For IgG1, IgG2b and IgG3 reactivity was less than 5% against target (IgG2a Heavy Chain, IgG).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:15000 - 1:30000
  • Flow Cytometry
  • Fluorophore-linked immunosorbent assay
  • Immunohistochemistry 1:2000 - 1:10000
  • Western Blot 1:4000 - 1:20000
• Application Notes: This product has been tested by ELISA, dot blot, and western blot and is suitable for immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG2a Heavy Chain Antibody (NBP1-72794)(2)

Publications using NBP1-72794

• Flood MD, Wellington AJ, Cruz LA, Eggers ED Early diabetes impairs ON sustained ganglion cell light responses and adaptation without cell death or dopamine insensitivity Exp Eye Res 2020-09-10 [PMID: 32910942] (IF/IHC, Mouse)
  
  Details:
  Citation using the DyLight 550 version of this antibody.
• Kim H, Kang K, Bang Y et al. MERS-CoV Spike Protein Vaccine and Inactivated Influenza Vaccine Formulated with Single Strand RNA Adjuvant Induce T-Cell Activation through Intranasal Immunization in Mice. Pharmaceutics 2020-05-10 [PMID: 32397649] (ELISA)
  
  Details:
  Citation using the HRP format of this antibody.