Rabbit anti-Mouse IgG2a Secondary Antibody [Fluorescein]

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Western Blot: Rabbit anti-Mouse IgG2A Secondary Antibody [Fluorescein] [NBP1-73637] - Lane 1: Mouse IgG2a. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein rabbit secondary ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, FLISA, Flow, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Fluorescein
Concentration
LYOPH

Rabbit anti-Mouse IgG2a Secondary Antibody [Fluorescein] Summary

Immunogen
Mouse IgG2a heavy chain
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) against a panel of immobilized mouse and human serum proteins resulting in a conjugated antibody designed for flow cytometry. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 - 1:10000
  • Flow Cytometry 1:500 - 1:2500
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 528, Excitation Wavelength: 495, Label Molecular Weight: 390, Restore with 1.0 mL of deionized water (or equivalent).

Alternate Names for Rabbit anti-Mouse IgG2a Secondary Antibody [Fluorescein]

  • IgG2A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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