Rabbit anti-Mouse IgG2A Secondary Antibody [Fluorescein]


Western Blot: Rabbit anti-Mouse IgG2A Secondary Antibody [Fluorescein] [NBP1-73637] - Lane 1: Mouse IgG2a. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein rabbit secondary ...read more

Product Details

Reactivity MuSpecies Glossary
Applications WB, FLISA, Flow, ICC/IF
LYOPH mg/ml

Order Details

Rabbit anti-Mouse IgG2A Secondary Antibody [Fluorescein] Summary

Mouse IgG2a heavy chain
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG2a coupled to agarose beads followed by solid phase adsorption(s) against a panel of immobilized mouse and human serum proteins resulting in a conjugated antibody designed for flow cytometry. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C in the dark.
Fluorescein (A=488, E=515-545)
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
0.01% Sodium Azide
LYOPH mg/ml
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.


  • Western Blot 1:1000 - 1:10000
  • Flow Cytometry 1:500 - 1:2500
  • Fluorophore-linked immunosorbent assay 1:10000 - 1:50000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000


Emission Wavelength: 528, Excitation Wavelength: 495, Label Molecular Weight: 390, Restore with 1.0 mL of deionized water (or equivalent).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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