Rabbit anti-Mouse IgG2a Secondary Antibody

Images

 
Western Blot: Rabbit anti-Mouse IgG2a Secondary Antibody [NBP1-72794] - Rabbit anti-Mouse IgG2A Secondary Antibody [NBP1-72794] - Lane 1: Mouse IgG1. Lane 2: Mouse IgG2a. Lane 3: Mouse IgG2b. Lane 4: Mouse IgG3. Lane 5: ...read more
Flow Cytometry: Rabbit anti-Mouse IgG2a Secondary Antibody [NBP1-72794] - Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Antibody [DyLight 405] [NBP1-72921]close
Western Blot: Rabbit anti-Mouse IgG2a Secondary Antibody [NBP1-72794] - Lane 1: Mouse IgG2a. Lane 2: none. Load: 50ng per lane. Primary antibody: none. Secondary antibody: Mouse IgG2a Antibody Alkaline Phosphatase ...read more
Dot Blot: Rabbit anti-Mouse IgG2a Secondary Antibody [NBP1-72794] - Mouse IgG2a Load: Lane 1 - 200ng Lane 2 - 66.7ng Lane 3 - 22.2ng Lane 4 - 7.4 Lane 2.5ng
Fluorophore-linked immunosorbent assay: Rabbit anti-Mouse IgG2a Secondary Antibody [NBP1-72794] - Rabbit anti-Mouse IgG2a Antibody [DyLight 800] [NBP1-72915]

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Rabbit anti-Mouse IgG2a Secondary Antibody Summary

Immunogen
Mouse IgG2a heavy chain
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1% cross-reactivity against other mouse heavy or light chain isotypes.
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:4000 - 1:20000
  • ELISA 1:15000 - 1:30000
  • Immunohistochemistry 1:2000 - 1:10000
  • Immunohistochemistry-Paraffin 1:2000 - 1:10000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Rabbit anti-Mouse IgG2a Secondary Antibody

  • IgG2A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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