IgG1 Antibodies

MAB3286, Mouse IgG_2Bisotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG_2Bisotype), anti-GAPDH (Catalog # MAB5718,Mouse IgG₁isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG₃isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG_2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG₁(Catalog # MAB9627) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgG₁isotype antibody at approximately 39 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) but not Rabbit Anti-Mouse IgG₁Monoclonal Antibody (Catalog # MAB9627). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> MAB3286, Mouse IgG_2Bisotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG_2Bisotype), anti-GAPDH (Catalog # MAB5718,Mouse IgG₁isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG₃isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG_2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG₁(Catalog # MAB9627) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgG₁isotype antibody at approximately 39 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) but not Rabbit Anti-Mouse IgG₁Monoclonal Antibody (Catalog # MAB9627). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 and Jurkat cell lines. PVDF membrane was probed with anti-HSP90 (Catalog # MAB3286, Mouse IgG_2Bisotype), anti-HSP60 (Catalog # MAB1800, Mouse IgG_2Bisotype), anti-GAPDH (Catalog # MAB5718,Mouse IgG₁isotype), anti-Peroxiredoxin 6 (Catalog # MAB3490, Mouse IgG₃isotype), and anti-SOD1 (Catalog # MAB3418, Mouse IgG_2Aisotype). The left panel was then probed with 0.5 µg/mL of Rabbit Anti-Mouse IgG₁(Catalog # MAB9627) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgG₁isotype antibody at approximately 39 kDa (as indicated). The right panel was probed with HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007) but not Rabbit Anti-Mouse IgG₁Monoclonal Antibody (Catalog # MAB9627). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB100, Mouse IgG₁isotype), Mouse anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG_2Aisotype), or Mouse anti-Human CD8a Monoclonal Antibody (Catalog # MAB1509, Mouse IgG_2Bisotype), followed by Rabbit anti-Mouse IgG₁-specific secondary antibody (Catalog # MAB9627) then PE-conjugated Goat anti-Rabbit secondary antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins." class="big_lightbox"> MAB100, Mouse IgG₁isotype), Mouse anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG_2Aisotype), or Mouse anti-Human CD8a Monoclonal Antibody (Catalog # MAB1509, Mouse IgG_2Bisotype), followed by Rabbit anti-Mouse IgG₁-specific secondary antibody (Catalog # MAB9627) then PE-conjugated Goat anti-Rabbit secondary antibody (Catalog # F0110). View our protocol for Staining Membrane-associated Proteins." class="big_thumb" />

HAF008). A specific band was detected for IgG₁at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for IgG₁at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of purified human IgG₁, purified human IgG₂(negative control), purified human IgG₃(negative control), and human purified human IgG₄(negative control). PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human IgG₁Monoclonal Antibody (Catalog # MAB9930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IgG₁at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

HAF018). A specific band was detected for IgG₁at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for IgG₁at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of purified human IgG₁, purified human IgG₂(negative control), purified human IgG₃(negative control), and human purified human IgG₄(negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IgG₁Monoclonal Antibody (Catalog # MAB9894) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IgG₁at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox"> DY990). Rabbit Anti-Human IgG Monoclonal Antibody (Catalog # MAB11012) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_thumb" />

HAF018). A specific band was detected for IgG₁at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). A specific band was detected for IgG₁at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of purified human IgG₁, purified human IgG₂(negative control), purified human IgG₃(negative control), and human purified human IgG₄(negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IgG₁Monoclonal Antibody (Catalog # MAB98941) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IgG₁at approximately 240 kDa (as indicated). Human IgG (Catalog # MAB1101) is shown as a loading control.This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />