Human IgG1 ELISA Kit (Chemiluminescence)

ELISA: Human IgG1 ELISA Kit (Chemiluminescence) [NBP3-00401] - Samples were spiked with high concentrations of Human IgG1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human IgG1 ELISA Kit (Chemiluminescence) [NBP3-00401] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.31 - 20 ug/mL
• Sensitivity: 0.19 ug/mL

Human IgG1 ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Human IgG1 in samples. No significant cross-reactivity or interference between Human IgG1 and analogues was observed.
• Standard Curve Range: 0.31 - 20 ug/mL
• Sensitivity: 0.19 ug/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: 4.6%
• Intra-Assay: 4.68%
• Spike Recovery: 86 - 106%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Chemiluminescence)

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IgG1 ELISA Kit (Chemiluminescence)

• IgG1
• Igh-4
• RGD1359539
• VH7183

Background

IgG is a monomeric immunoglobulin composed of two heavy chains and two light chains. There are four subclasses of the IgG: IgG1, IgG2, IgG3 and IgG4. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin as well as the only isotype that can pass through the placent,a thereby providing protection to the fetus in its first weeks of life before, its own immune system has developed. IgG can bind to several different kinds of pathogens, for example viruses, bacteria and fungi, and it protects the body against them by complement activation (the classic pathway), opsonization for phagocytosis and neutralization of their toxins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IgG1 ELISA Kit (NBP3-00401). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.