Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 594]

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Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, ELISPOT
Clonality
Polyclonal
Host
Donkey
Conjugate
DyLight 594

Order Details

Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 594] Summary

Immunogen
This Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) was developed against goat IgG whole molecule.
Specificity
Assay of Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-adsorbed) by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey serum, Goat IgG and Goat serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit or Rat serum proteins. Specificity was confirmed using ELISA minimal cross reactivity against the targets stated above. This antibody was pre-adsorbed against chicken guinea pig hamster horse mouse rabbit & rat serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot : 1:500-1:2000
  • ELISA : 1:20000 - 1:100000
  • Immunohistochemistry : 1:200-1:1000
  • Immunohistochemistry-Frozen : 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin : 1:1000 - 1:5000
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Background

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NBP2-60657DL594) (0)

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Other Available Formats

Alkaline Phosphatase NB120-7126
Allophycocyanin/Cy7 NBP2-60657APCCY7
Biotin NB120-7124
DyLight 350 NBP2-60657UV
DyLight 405 NBP1-72853
DyLight 488 NBP1-72843
DyLight 549 NBP1-72845
DyLight 550 NBP2-60657R
DyLight 594 NBP2-60657DL594
DyLight 649 NBP1-72847
DyLight 650 NBP2-60657C
DyLight 680 NBP1-72849
DyLight 755 NBP2-60657IR
FITC NBP1-73566
HRP NBP1-73714
Janelia Fluor 549 NBP2-60657JF549
Janelia Fluor 646 NBP2-60657JF646
PE/Atto594 NBP2-60657PEATT594
PE/Cy5.5 NBP2-60657PECY55
PE/Cy7 NBP2-60657PECY7
Rhodamine NB120-7122
Texas Red NB120-7123

Additional IgG (H+L) Products

Array NBP2-60657DL594

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