Donkey anti-Goat IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed)

Images

 
Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP2-60657] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at ...read more
Immunocytochemistry/ Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP2-60657] - Image shows detection of PD-1 in mouse frozen tissue sections (salivary gland injected with human T ...read more
Flow Cytometry: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP2-60657] - Fluorophore-linked immunosorbent assay: Donkey anti-Goat IgG, H/L Chains Antibody [DyLight 649] [NBP1-72847] Image using the ...read more
Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP2-60657] - Lane 1: Goat IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Texas Red donkey secondary ...read more
Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP2-60657] - Rabbit anti-Transferrin, Goat-anti-Alpha-1-Anti-Trypsin, and Mouse-a-GST. Detection shown was using DyLight549 Donkey anti-Rabbit ...read more
Immunocytochemistry/ Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP2-60657] - Image shows anti-Histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was ...read more
Flow Cytometry: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP2-60657] - Fluorophore-linked immunosorbent assay: Donkey anti-Goat IgG, H/L Chains Antibody [DyLight 405] [NBP1-72853] Image using the ...read more
Flow Cytometry: Donkey anti-Goat IgG (H+L) Secondary Antibody (Pre-absorbed) [NBP2-60657] - Fluorophore-linked immunosorbent assay: Donkey anti-Goat IgG, H/L Chains Antibody [DyLight 549] [NBP1-72845] Image using the ...read more

Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Donkey
Conjugate
Unconjugated
Format
Pre-adsorbed
Concentration
1 mg/ml

Donkey anti-Goat IgG (H+L) Secondary Antibody [Unconjugated] (Pre-adsorbed) Summary

Immunogen
Goat IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit or Rat Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Frozen 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
Application Notes
This product is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency.
Publications
Read Publications using
NBP2-60657 in the following applications:

  • 1 publication
  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP2-60657)(3)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Additional Array Products

Array NBP2-60657

Blogs on IgG (H+L)

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