Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed)

Images

 
Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72843] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at ...read more
Immunocytochemistry/ Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72843] - Image shows anti-Histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was ...read more
Immunohistochemistry-Frozen: Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72843] - Image shows detection of PD-1 in mouse frozen tissue sections (salivary gland injected with human T cells) using ...read more
Fluorophore-linked immunosorbent assay: Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72843]

Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, FLISA, ICC/IF
Clonality
Polyclonal
Host
Donkey
Conjugate
DyLight 488
Format
Pre-absorbed
Concentration
LYOPH

Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) Summary

Immunogen
Goat IgG, whole molecule
Specificity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins. This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-72843 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Notes

Emission Wavelength: 518, Excitation Wavelength: 493, Label Molecular Weight: 1,011, Restore with 0.1 mL of deionized water (or equivalent).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP1-72843) (0)

There are no publications for IgG (H+L) Antibody (NBP1-72843).
By submitting your publication information earn gift cards and discounts for future purchases.

Review for IgG (H+L) Antibody (NBP1-72843) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: IF.

Reviews using NBP1-72843:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence IgG (H+L) NBP1-72843
Enlarge
reviewed by:
Christopher England
IF Mouse 07/09/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse salivary gland injected with human T cells
SpeciesMouse
Lot27302

Blocking

Blocking Details10% Normal Donkey Serum, 0.25% Triton-X in PBS, 1 hr at room temperature

Primary Anitbody

Dilution RatioPD-1 Primary and CD3 Primary Antibodies, 1:200, 12 h, 4 degree Celcius, PBS w/0.25% Triton-X

Secondary Antibody

Secondary DescriptionDonkey anti-Goat DyLight 488
Secondary Manufacturer Cat#NBP1-72843
Secondary Concentration1:4000

Details

Detection NotesConfocal microscopy, Nikon A1R
Fixation DetailsFrozen Sections, 4% Paraformaldehyde in PBS for 30 min at room temperature
Wash DescriptionPBS, 15 min, 3-times after primary and 3-times after secondary

Comments

CommentsThis secondary antibody worked great. We got the results that we expected and the staining looks good for trying to image a biomarker that is lowly expressed.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NBP1-72843) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional IgG (H+L) Products

Blogs on IgG (H+L)

There are no specific blogs for IgG (H+L), but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Christopher England
07/09/2016
Application: IF
Species: Mouse