Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] (Pre-adsorbed)

Images

 
Western Blot: Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72843] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated ...read more
Immunocytochemistry/ Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72843] - Image shows anti-Histone detection using a DyLight™ 488 conjugate (green). ...read more
Immunohistochemistry-Frozen: Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72843] - Image shows detection of PD-1 in mouse frozen tissue sections (salivary gland injected with human T ...read more
Fluorophore-linked immunosorbent assay: Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] (Pre-absorbed) [NBP1-72843]

Product Details

Summary
Reactivity GtSpecies Glossary
Applications WB, FLISA, ICC/IF, IHC-Fr
Clonality
Polyclonal
Host
Donkey
Conjugate
DyLight 488
Format
Pre-adsorbed
Concentration
LYOPH

Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] (Pre-adsorbed) Summary

Immunogen
Goat IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:10000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-72843 in the following applications:

Publications
Read Publication using
NBP1-72843 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 100 ul of deionized water.

Notes

Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IgG (H+L) Antibody (NBP1-72843)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for IgG (H+L) Antibody (NBP1-72843) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-72843:
Filter by Applications
IF
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunofluorescence IgG (H+L) NBP1-72843
Enlarge
reviewed by:
Christopher England
IF Mouse 07/09/2016
View

Summary

ApplicationImmunofluorescence
Sample TestedMouse salivary gland injected with human T cells
SpeciesMouse
Lot27302

Blocking

Blocking Details10% Normal Donkey Serum, 0.25% Triton-X in PBS, 1 hr at room temperature

Primary Anitbody

Dilution RatioPD-1 Primary and CD3 Primary Antibodies, 1:200, 12 h, 4 degree Celcius, PBS w/0.25% Triton-X

Secondary Antibody

Secondary DescriptionDonkey anti-Goat DyLight 488
Secondary Manufacturer Cat#NBP1-72843
Secondary Concentration1:4000

Details

Detection NotesConfocal microscopy, Nikon A1R
Fixation DetailsFrozen Sections, 4% Paraformaldehyde in PBS for 30 min at room temperature
Wash DescriptionPBS, 15 min, 3-times after primary and 3-times after secondary

Comments

CommentsThis secondary antibody worked great. We got the results that we expected and the staining looks good for trying to image a biomarker that is lowly expressed.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NBP1-72843) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Array Products

Array NBP1-72843

Blogs on IgG (H+L)

There are no specific blogs for IgG (H+L), but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Christopher England
07/09/2016
Application: IF
Species: Mouse