Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488]


Western Blot: Donkey anti-Goat IgG, H/L Chains Antibody [DyLight 488] [NBP1-72843] - Lane 1: Goat IgG Load: 50 ug per lane Secondary antibody: Goat IgG (H&L) Antibody DyLight (TM) 488 conjugated Pre-Adsorbed at 1:1,000 more
Immunocytochemistry/ Immunofluorescence: Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72843] - Image shows anti-Histone detection using a DyLight™ 488 conjugate (green). Anti-Tubulin was more
Immunohistochemistry-Frozen: Donkey anti-Goat IgG (H+L) Secondary Antibody [DyLight 488] [NBP1-72843] - Image shows detection of PD-1 in mouse frozen tissue sections (salivary gland injected with human T cells) using more
Fluorophore-linked immunosorbent assay: Donkey anti-Goat IgG, H/L Chains Antibody [DyLight 488] [NBP1-72843]

Product Details

Reactivity GtSpecies Glossary
Applications WB, FLISA, ICC/IF
LYOPH mg/ml

Order Details

Donkey anti-Goat IgG (H L) Secondary Antibody [DyLight 488] Summary

Goat IgG, whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins. This antibody will react with heavy chains of Goat IgG and with light chains of most Goat immunoglobulins.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
DyLight 488
DyLight 488 (A=493, E=518)
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/ml BSA - Immunoglobulin and Protease free
0.01% Sodium Azide
LYOPH mg/ml
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.


  • Western Blot 1:10000
  • Fluorophore-linked immunosorbent assay 1:20000
  • Immunocytochemistry/Immunofluorescence 1:5000
Application Notes
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Reviewed Applications
Read 1 Review rated 5
NBP1-72843 in the following applications:


Emission Wavelength: 518, Excitation Wavelength: 493, Label Molecular Weight: 1,011, Restore with 0.1 mL of deionized water (or equivalent).

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Reviews for IgG (H+L) Antibody (NBP1-72843) (1) 51

Average Rating: 5
(Based on 1 reviews)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-72843:
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Images Ratings Applications Species Date Details
 IgG (H+L) NBP1-72843
reviewed by:
Mouse 07/09/2016


Sample TestedMouse salivary gland injected with human T cells


Blocking Details10% Normal Donkey Serum, 0.25% Triton-X in PBS, 1 hr at room temperature

Primary Anitbody

Dilution RatioPD-1 Primary and CD3 Primary Antibodies, 1:200, 12 h, 4 degree Celcius, PBS w/0.25% Triton-X

Secondary Antibody

Secondary DescriptionDonkey anti-Goat DyLight 488
Secondary Manufacturer Cat#NBP1-72843
Secondary Concentration1:4000


Detection NotesConfocal microscopy, Nikon A1R
Fixation DetailsFrozen Sections, 4% Paraformaldehyde in PBS for 30 min at room temperature
Wash DescriptionPBS, 15 min, 3-times after primary and 3-times after secondary


CommentsThis secondary antibody worked great. We got the results that we expected and the staining looks good for trying to image a biomarker that is lowly expressed.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IgG (H+L) Antibody (NBP1-72843) (0)

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