Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed)

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Western Blot: Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NBP1-75290] - analysis of PP4C in Huh4 cell lysate. Image from verified customer review.

Product Details

Summary
Reactivity RbSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Donkey
Conjugate
HRP
Format
Pre-absorbed
Concentration
LYOPH

Donkey anti-Rabbit IgG (H+L) Secondary Antibody [HRP] (Pre-absorbed) Summary

Immunogen
Purified Rabbit IgG (H&L)
Specificity
Based on Immunoelectrophoresis, this antibody reacts with heavy (gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins. Cross Adsorbed: This antibody was pre-adsorbed to reduce cross-reactivity with proteins of non-related species. Minimal cross-reactivity with bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG serum proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Donkey
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:200-1:5000
  • ELISA 1:200-1:5000
  • Immunohistochemistry 1:500-1:5000
Application Notes
This conjugate is suitable for all immunoassay applications.
Readout System
Reviewed Applications
Read 2 Reviews rated 5
using
NBP1-75290 in the following applications:

Reactivity Notes

Based on IEP, this antibody reacts with heavy (gamma) chains on rabbit IgG and light chains on all rabbit immunoglobulins.

Packaging, Storage & Formulations

Storage
Store at 4C. After reconstitution, dilute with 50% glycerol and store at -20C. Avoid repeated freeze-thaw cycles.
Buffer
10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free.
Preservative
0.1% Kathon
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 1.1 ml deionized water and let stand 30 minutes at RT to disolve. Centrifuge to remove any particulates.

Notes

Purity > 95% based on SDS-PAGE. Donkey serum was obtained from healthy animals of US origin and under the care of a registered veterinarian. After reconstituted, prepare fresh working dilution daily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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Reviews for Rabbit IgG, H/L Chains Antibody (NBP1-75290) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.
We have 2 reviews tested in 1 application: WB.

Reviews using NBP1-75290:
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WB
(2)
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Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Sharon Miksys
WB Other 03/11/2015
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Summary

ApplicationWestern Blot
Sample TestedLiver, Brain
SpeciesOther

Blocking

Blocking Details5% (w/v) skim milk, 0.1% (w/v) BSA, 1% (v/v) normal horse serum, 2 hours, room temperature

Secondary Antibody

Secondary DescriptionDonkey anti-Rabbit IgG, H/L Chains Antibody [HRP]
Secondary Manufacturer Cat#NBP1-75290
Secondary Concentration1:5,000 to 1:15,000

Details

Detection NotesECL, 30 seconds to 5 minutes exposure

Comments

CommentsGreat sensitivity on liver and brain samples previously probed with monoclonal or polyclonal rabbit primary antibodies. Did not detect unspecific signal when it was used to probe liver and brain samples in absence of primary Ab.
Western Blot IgG (H+L) NBP1-75290
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reviewed by:
Anonymous
WB Human 11/12/2014
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Summary

ApplicationWestern Blot
Sample TestedHuh7 whole cell lysate
SpeciesHuman

Blocking

Blocking Detailsblocked in 5% milk, 1 hr at RT

Primary Anitbody

Dilution Ratioprimary antibody against PP4C diluted 1/300 in 5% milk, 1 hr at RT

Secondary Antibody

Secondary Descriptionthis is the secondary

Details

Detection Notesfilm, exposed for 120 seconds

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FAQs for Rabbit IgG, H/L Chains Antibody (NBP1-75290) (0)

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Recent Reviews

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Sharon Miksys
03/11/2015
Application: WB
Species: Other

Anonymous
11/12/2014
Application: WB
Species: Human