Donkey anti-Rabbit IgG (H+L) Secondary Antibody [Biotin]


Western Blot: Donkey anti-Rabbit IgG Secondary Antibody [Biotin] [NB120-6801] - Lane M: 3 ul Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment. Lane more

Product Details

Reactivity RbSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Please see the vial label for concentration. If unlisted please contact technical services.

Order Details

Donkey anti-Rabbit IgG (H L) Secondary Antibody [Biotin] Summary

Rabbit IgG whole molecule
Rabbit IgG (H and L)
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Biotin (A=N/A, E=N/A)
0.02M K phosphate, 0.15M NaCl, pH7.2 with 10mg/ml BSA.
0.05% Sodium Azide
Immunogen affinity purified


  • Western Blot 1:3000 - 1:15000
  • ELISA 1:300000
  • Immunohistochemistry 1:500 - 1:3000
  • Immunohistochemistry-Paraffin
Readout System

Reactivity Notes



Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10^11 variants.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol

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