Rabbit F(ab')2 anti-Guinea Pig IgG (H+L) Secondary Antibody [Biotin] (Pre-absorbed)

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Product Details

Summary
Reactivity GPSpecies Glossary
Applications WB, ELISA, IHC, IHC-Fr, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin
Format
Pre-absorbed
Concentration
LYOPH

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Rabbit F(ab')2 anti-Guinea Pig IgG (H+L) Secondary Antibody [Biotin] (Pre-absorbed) Summary

Immunogen
Guinea pig IgG whole molecule
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum, Guinea Pig IgG and Guinea Pig Serum. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:90000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Frozen 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin 1:1000 - 1:5000
Application Notes
This product is designed for ICC/IF, FLISA and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Readout System

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with 500 ul of deionized water.

Notes

Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in human serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 10^11 variants.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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