Rabbit F(ab')2 anti-Rat IgG (H+L) Secondary Antibody [Biotin]


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Product Details

Reactivity RtSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
1 mg/ml

Order Details

Rabbit F(ab')2 anti-Rat IgG (H L) Secondary Antibody [Biotin] Summary

Rat IgG whole molecule
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Rat IgG and Rat Serum. No reaction was observed against anti-Pepsin, anti-Rabbit IgG F(c) or Bovine, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
Immunogen affinity purified

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Biotin (A=N/A, E=N/A)
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/ml Bovine Serum Albumin (BSA)
0.05% Sodium Azide
1 mg/ml
Immunogen affinity purified


  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000 - 1:100000
  • Immunohistochemistry 1:1000 - 1:5000
  • Immunohistochemistry-Paraffin
Application Notes
This product has been assayed against 1.0 ug of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS ELISA PEROXIDASE SUBSTRATE for 30 minutes at room temperature. A working dilution of 1:10,000 - 1:50,000 of the reconstitution concentration is suggested for this product.
Readout System

Reactivity Notes

Cross-reacts with Rat.Does not cross-react with Cow, Horse, Human, Mouse, Rabbit or Sheep.Not yet tested in other species.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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WB Video Protocol

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