Equine IgG Fc Isotype Control [HRP]

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Product Details

Summary
Reactivity EqSpecies Glossary
Applications WB, ELISA
Host
Horse
Conjugate
HRP
Concentration
LYOPH

Order Details

Equine IgG Fc Isotype Control [HRP] Summary

Description
This Equine IgG Fc immunoglobulin is useful as an isotype control for Equine IgG Fc antibody.
Specificity
No reaction was observed against anti-Horse IgG F(ab')2 or anti-Papain.
Isotype
IgG
Host
Horse
Purity
Multi-step

Applications/Dilutions

Dilutions
  • ELISA
  • Western Blot
Application Notes
This isotype control can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.

NOTE: Do NOT add Sodium Azide (it inhibits HRP irreversibly).

Packaging, Storage & Formulations

Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Gentamicin Sulfate
Concentration
LYOPH
Purity
Multi-step
Reconstitution Instructions
Reconstitute with 1.0 ml deionized water (or equivalent).

Notes

This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Horse IgG, anti-Horse IgG F(c) and anti-Horse Serum

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Isotype Controls are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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