Horse IgG, Fc Native Protein

SDS-Page: Horse IgG, Fc Native Protein [NBP1-97034] - Lane 1: Horse IgG Fragment Reduced. Lane 2: Horse IgG Fragment Non reduced. Load: 1.0ug per lane.

• Reactivity: Eq
• Applications: WB, ELISA, PAGE

Horse IgG, Fc Native Protein Summary

• Description: Native Equine Horse IgG, Fc
  
  Source: Normal serum
  
  Store vial at 4C prior to opening. This product is stable 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
  
  Prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis.
• Specificity: Assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum, anti-Horse IgG and anti-Horse IgG F(c). No reaction was observed against anti-Horse IgG F(ab')2 or anti-Papain.
• Preparation Method: This product was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Horse IgG F(c) fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Horse Serum, anti-Horse IgG and anti-Horse IgG F(c). No reaction was observed against anti-Horse IgG F(ab')2 or anti-Papain.
• Protein/Peptide Type: Native Protein
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA
  • SDS-Page
  • Western Blot
• Application Notes: This product has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments. Suggested application: IHC.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Background

Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(c) region, recognized by high-affinity Fc receptor proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.