Goat anti-Rat IgE Fc Secondary Antibody

• Reactivity: Rt
• Applications: WB, DB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Goat anti-Rat IgE Fc Secondary Antibody Summary

• Description: The lyophilized IgG fraction is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at 4C, not refrozen, and preferably used the same day.
• Immunogen: Pools of purified homogenous IgE isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure
• Specificity: The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of IgE, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen. It does not react with rat IgG, including all subclasses, IgA, IgM and IgG/Fab or any non-Ig protein in rat serum.
• Clonality: Polyclonal
• Host: Goat
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Western Blot
• Application Notes: Indirect immunofluorescence.

Reactivity Notes

This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this conjugate has not been tested in detail

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.2)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Ion exchange chromatography
• Reconstitution Instructions: Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2).

Alternate Names for Goat anti-Rat IgE Fc Secondary Antibody

• constant region of heavy chain of IgE
• IgE Fc
• immunoglobulin epsilon
• immunoglobulin heavy constant epsilon

Background

Predicted to enable antigen binding activity and immunoglobulin receptor binding activity. Predicted to be involved in several processes, including activation of immune response; defense response to other organism; and phagocytosis. Predicted to be located in extracellular region. Predicted to be part of immunoglobulin complex, circulating. Predicted to be active in external side of plasma membrane. [provided by Alliance of Genome Resources, Apr 2022]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.