Goat anti-Rat IgE Fc Secondary Antibody [TRITC]

• Reactivity: Rt
• Applications: ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: TRITC
• Concentration: LYOPH

Goat anti-Rat IgE Fc Secondary Antibody [TRITC] Summary

• Description: The lyophilized conjugate is shipped at ambient temperature and may be stored at 4C; prolonged storage at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
• Immunogen: Pools of purified homogenous IgE isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure
• Specificity: The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule as tested by gel immunodiffusion techniques against rat sera and a panel of purified homogenous immunoglobulins. A reaction of full identity is obtained with serum containing monoclonal IgE and with the immunogen. No reaction is obtained with normal rat serum. The product does not react with any other protein of rat plasma or serum.
• Clonality: Polyclonal
• Host: Goat
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 1:10 - 1:500
• Application Notes: (In)direct immunofluorescence.

Reactivity Notes

This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this conjugate has not been tested in detail

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: Lyophilized from PBS (pH 7.2)
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Ion exchange chromatography
• Reconstitution Instructions: Reconstitute by by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20C.

Alternate Names for Goat anti-Rat IgE Fc Secondary Antibody [TRITC]

• constant region of heavy chain of IgE
• IgE Fc
• immunoglobulin epsilon
• immunoglobulin heavy constant epsilon

Background

Predicted to enable antigen binding activity and immunoglobulin receptor binding activity. Predicted to be involved in several processes, including activation of immune response; defense response to other organism; and phagocytosis. Predicted to be located in extracellular region. Predicted to be part of immunoglobulin complex, circulating. Predicted to be active in external side of plasma membrane. [provided by Alliance of Genome Resources, Apr 2022]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.