Human Respiratory Syncytial Virus (RSV) nucleoprotein VPN41 Antibody (4-18)

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Western Blot: Respiratory Syncytial Virus Nucleoprotein Antibody (RSV 4-18) [NBP2-50535]

Product Details

Summary
Reactivity ViSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
4-18
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Human Respiratory Syncytial Virus (RSV) nucleoprotein VPN41 Antibody (4-18) Summary

Immunogen
Gradient-purified RSN-2 virus (subgroup B) treated with 0.1% SDS at 100C for 2 mins.
Specificity
This antibody reacts with both human RSV subgroups A and B.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Purity
Protein G purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500

Reactivity Notes

Human Respiratory Syncytial Virus (RSV).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Notes

Positive control(s): Immunoblot: Gradient-purified RSN-2 virus 5ug per lane. Lanes 1 and 2.First antibodies: Line1 RSV convalescent human sera. Lane 2: 4-18 antibody. Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells.

Background

Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children. There are two RS virus subgroups, A and B. The A and B subgroups circulate concurrently with subgroup A usually dominating. This antibody is useful for the diagnosis of RS virus subgroup A and B infections, since the nucleoprotein is very abundant within infected cells, which makes virus detection assays highly sensitive.The nucleoprotein is located in the nucleocapsid of the virion; it is abundant and forms a tight complex with virus genomic RNA. Anti-Nucleoprotein VP41 [RSV 4-18] reacts with both human RS virus Subgroups A and B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Respiratory Syncytial Virus Nucleoprotein Antibody (NBP2-50535) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-50535AF350
Alexa Fluor 405 NBP2-50535AF405
Alexa Fluor 488 NBP2-50535AF488
Alexa Fluor 532 NBP2-50535AF532
Alexa Fluor 594 NBP2-50535AF594
Alexa Fluor 647 NBP2-50535AF647
Alexa Fluor 700 NBP2-50535AF700
Alexa Fluor 750 NBP2-50535AF750
Biotin NBP2-50535B
DyLight 350 NBP2-50535UV
DyLight 405 NBP2-50535V
DyLight 488 NBP2-50535G
DyLight 550 NBP2-50535R
DyLight 594 NBP2-50535DL594
DyLight 650 NBP2-50535C
DyLight 680 NBP2-50535FR
DyLight 755 NBP2-50535IR
FITC NBP2-50535F
HRP NBP2-50535H
Janelia Fluor 549 NBP2-50535JF549
Janelia Fluor 646 NBP2-50535JF646
PerCP NBP2-50535PCP

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