Human Respiratory Syncytial Virus Nucleoprotein VPN41 Antibody (4-18) - BSA Free

Western Blot: Human Respiratory Syncytial Virus Nucleoprotein VPN41 Antibody (4-18) - BSA Free [NBP2-50535] - Western Blot: Human Respiratory Syncytial Virus Nucleoprotein VPN41 antibody

• Reactivity: V-Vi
• Applications: WB, ELISA, ICC/IF
• Clone: 4-18
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Human Respiratory Syncytial Virus Nucleoprotein VPN41 Antibody (4-18) - BSA Free Summary

• Immunogen: Gradient-purified RSN-2 virus (subgroup B) treated with 0.1% SDS at 100C for 2 mins.
• Specificity: This antibody reacts with both human RSV subgroups A and B.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Western Blot 1:100 - 1:2000

Reactivity Notes

Human Respiratory Syncytial Virus (RSV).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Notes

Positive control(s): Immunoblot: Gradient-purified RSN-2 virus 5ug per lane. Lanes 1 and 2.First antibodies: Line1 RSV convalescent human sera. Lane 2: 4-18 antibody. Indirect immunofluorescence: staining of RSN-2 infected BSC-1 cells.

Background

Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children. There are two RS virus subgroups, A and B. The A and B subgroups circulate concurrently with subgroup A usually dominating. This antibody is useful for the diagnosis of RS virus subgroup A and B infections, since the nucleoprotein is very abundant within infected cells, which makes virus detection assays highly sensitive.The nucleoprotein is located in the nucleocapsid of the virion; it is abundant and forms a tight complex with virus genomic RNA. Anti-Nucleoprotein VPN41 [RSV 4-18] reacts with both human RS virus Subgroups A and B.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.