Human IgM Antibody (ICL-931) - Azide and BSA Free

• Reactivity: Hu
• Applications: WB, DB, ELISA, Func, IHC
• Clone: ICL-931
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

Human IgM Antibody (ICL-931) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Human IgM Antibody (ICL-931) - Azide and BSA Free (NBP1-42268) is a monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Purified human IgM
• Specificity: Reacts with human IgM, mu-chain. Does not react with IgA, IgE, IgG and other plasma proteins
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Radioimmunoassay 1:100-1:2000
  • Western Blot
• Application Notes: The antibody reacts with human IgM in ELISA, RIA and Dot blot. Useful to detect human IgM on frozen sections and to selectively deplete human IgM from plasma by means of immunoabsorption.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM Sodium Phosphate (pH 7.5), 0.15M NaCl and 1.0% Mannitol
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Ion exchange chromatography

Alternate Names for Human IgM Antibody (ICL-931) - Azide and BSA Free

• AGM1
• MU
• VH

Background

IgM is the largest by physical size of the immunoglobulins. It forms a pentamer of immunoglobulins linked by disulfide bonds and usually contains a J chain. It is the first antibody to be produced in response to an antigen and possesses high avidity and is effective at activating complement.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.