![Flow Cytometry: Human IgE Antibody (I27/mAb27) [DDX0290P-100] - Fixation of mAb27 to IgEbound to FcεR2/CD23 on RPMI 8866 cells](http://images.novusbio.com/fullsize/Human-IgE-Antibody-I27-mAb27-Flow-Cytometry-DDX0290P-100-img0001.jpg "Flow Cytometry: Human IgE Antibody (I27/mAb27) [DDX0290P-100] - Fixation of mAb27 to IgEbound to FcεR2/CD23 on RPMI 8866 cells")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, Func, B/N |
| Clone | I27/mAb27 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | 0.5 mg/ml |
| Immunogen | Human IgE was used as immunogen. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Ion exchange chromatography |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Tris-NaCl (pH 8.0) |
| Preservative | No Preservative |
| Concentration | 0.5 mg/ml |
| Purity | Ion exchange chromatography |
| Publication using DDX0290P-100 | Applications | Species |
|---|---|---|
| Chretien I et al. A monoclonal anti-IgE antibody against an epitope (amino acids 367-376) in the CH3 domain inhibits IgE binding to the low affinity IgE receptor (CD23). J Immunol 1988-01-01 [PMID: 2459242] |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Sandwich ELISA: Human IgE Antibody (I27/mAb27) [DDX0290P-100] - Bindingof mAb27 to IgE (open) and heat denatured IgE (closed) in a sandwich ELISA](http://images.novusbio.com/fullsize/Human-IgE-Antibody-I27-mAb27-Sandwich-ELISA-DDX0290P-100-img0002.jpg "Sandwich ELISA: Human IgE Antibody (I27/mAb27) [DDX0290P-100] - Bindingof mAb27 to IgE (open) and heat denatured IgE (closed) in a sandwich ELISA")
![Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB](https://images.novusbio.com/images/Goat-anti-Mouse-IgG-H+L-Secondary-Antibody-HRP-Immunohistochemistry-Paraffin-NB7539-img0014.jpg "Immunohistochemistry-Paraffin: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] [NB7539] - FFPE serial sections of human stomach carcinoma. Primary Antibody: Mouse anti-p53 (Clone DO-1) used at a dilution of 1:100. Secondary Antibody: Goat anti-Mouse IgG (H+L) Secondary Antibody [HRP] Conjugated used at a dilution of 1:200 (5ug/ml). Detection: DAB")
![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")