HSP65 (mycobacterial) Antibody (3F7)

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Western Blot: HSP65 (mycobacterial) Antibody (3F7) [NBP1-97880] - Analysis of HSP65: Lane 1: MW marker, Lane 2: GroEL Recombinant E. coli Protein Lane 3: HSP60 Recombinant Human Protein, Lane 4: HSP65 Recombinant ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ba, Bv, Dr, GP, Ha, PrSpecies Glossary
Applications WB
Clone
3F7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

HSP65 (mycobacterial) Antibody (3F7) Summary

Immunogen
Recombinant M. bovis HSP65.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
This antibody detects a band of ~65kDa by Western blot
Publications
Read Publication using NBP1-97880.

Reactivity Notes

Bacteria (E. coli, M. leprae, M. bovis), cryptosporidium Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for HSP65 (mycobacterial) Antibody (3F7)

  • 60 kDa chaperonin 2
  • 65 kDa antigen
  • Antigen A
  • Cell wall protein A
  • GroEL protein 2
  • groEL-2
  • groL2
  • Heat shock protein 65
  • hsp65
  • MT0456
  • MTV037.04
  • Protein Cpn60-2
  • Rv0440

Background

Hsp65 is a member of the Hsp60 family of heat shock proteins isolated from Mycobacterium bovis BCG. Hsp65 from M. bovis is identical to that of M. tuberculosis, and similar to that of M. leprae, two highly pathogenic strains of mycobacterium. Hsp65 is the immunodominant antigen during mycobacterial infection and vaccination, and has been linked to the development of autoimmune adjuvant arthritis in rats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HSP65 (mycobacterial) Antibody (NBP1-97880)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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