![Western Blot: Hsp40/YDJ1 Antibody (1G10.H8) [NBP2-12879] - Western Blot analysis of Yeast Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 1G10.H8 (NBP2-12879). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (NBP2-12879) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.](http://images.novusbio.com/fullsize2/Hsp40-YDJ1 Antibody (1G10.H8)-Western Blot-NBP2-12879-img0003.jpg "Western Blot: Hsp40/YDJ1 Antibody (1G10.H8) [NBP2-12879] - Western Blot analysis of Yeast Cell lysates showing detection of Hsp40 protein using Mouse Anti-Hsp40 Monoclonal Antibody, Clone 1G10.H8 (NBP2-12879). Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp40 Monoclonal Antibody (NBP2-12879) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.")
| Reactivity | YeSpecies Glossary |
| Applications | WB, ELISA, IP |
| Clone | 1G10.H8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Full length protein yeast HSP40 (YDJ1) |
| Localization | Cytoplasm , Nucleus |
| Specificity | Detects approx 40kDa. Yeast specific product. |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | YDJ1 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | 0.5 ug/ml of HSP40, YDJ1 Antibody was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | 50% Glycerol |
| Preservative | 0.09% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein G purified |
Secondary Antibodies |
Isotype Controls |
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| Gene Symbol | YDJ1 |
![Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.](https://images.novusbio.com/images2/Mouse-IgG1-Kappa-Light-Chain-Isotype-Control-(P3.6.2.8.1)-Flow-Cytometry-NBP1-43319-img0002.jpg "Flow Cytometry: Mouse IgG1 Kappa Isotype Control (P3.6.2.8.1) [NBP1-43319] - Analysis of Alexa Fluor (R) 700 conjugate of NBP1-43319. Human buffy coat stained with mouse IgG1 Kappa isotype control. Flow cytometry image submitted by a verified customer review.")