HRP Antibody (HP-03) - BSA Free

Images

 
Western Blot: HRP Antibody (HP-03) [NB500-420] - Purified horse radish peroxidase sample; non-reduced sample, immunostained by mAb HP-03 and anti-goat anti-mouse IgG (H+L)-HRP conjugate.

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
HP-03
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

HRP Antibody (HP-03) - BSA Free Summary

Immunogen
Horseradish peroxidase (Grade I).
Specificity
The antibody HP-03 reacts with horseradish peroxidase. It is suitable for PAP detection system.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Purity
Ammonium sulfate precipitation
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
  • Western Blot 1:1000
Application Notes
The antibody HP-03 was successfully used for staining of formaldehyde- fixed, Triton X- permeabilized cells transfected with HRP gene.
Publications
Read Publications using NB500-420.

Reactivity Notes

Detects HRP.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Purity
Ammonium sulfate precipitation

Alternate Names for HRP Antibody (HP-03) - BSA Free

  • EC 1.11.1.7
  • Horseradish Peroxidase
  • HPRC 1
  • HPRC1
  • HRPA2
  • HRPC 2
  • HRPC2
  • HRPE5
  • HRPN
  • Neutral peroxidase
  • Peroxidase A2
  • Peroxidase C1A
  • Peroxidase C1B
  • Peroxidase C2
  • Peroxidase C3
  • Peroxidase E5
  • Peroxidase N
  • PRXC1A
  • PRXC1B
  • PRXC2
  • PRXC3

Background

Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue. HRP binds 1 heme B (iron-protoporphyrin IX) group and 2 calcium ions per subunit. Subunits are monomeric

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HRP Antibody (NB500-420)(2)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HRP Antibody (NB500-420) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional HRP Products

Array NB500-420

Blogs on HRP.

Ultrasensitive IHC Detection with HRP-Polymer Conjugates
Signal amplification methods are widely used in immunohistochemistry (IHC) for detection of rare epitopes and low abundance antigens. While many of these techniques such as the avidin-biotin complex (ABC) method improve staining, they frequently re...  Read full blog post.

5 Stars for the Lightning-Link HRP Antibody Labeling Kit
Novus' Lightning-Link HRP Antibody Labeling Kit (cat# 701-0000) has received some glowing product reviews and customer feedback lately. One satisfied customer wrote, "Fast, efficient, stable direct HRP conjugation - best reagent we have found..." Anot...  Read full blog post.

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