![Western Blot: HRP Antibody (HP-03) [NB500-420] - Purified horse radish peroxidase sample; non-reduced sample, immunostained by mAb HP-03 and anti-goat anti-mouse IgG (H+L)-HRP conjugate.](http://images.novusbio.com/fullsize/HRP-Antibody-HP-03-Western-Blot-NB500-420-img0001.jpg "Western Blot: HRP Antibody (HP-03) [NB500-420] - Purified horse radish peroxidase sample; non-reduced sample, immunostained by mAb HP-03 and anti-goat anti-mouse IgG (H+L)-HRP conjugate.")
| Reactivity | All-NA, PlSpecies Glossary |
| Applications | WB, ELISA, ICC/IF |
| Clone | HP-03 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Immunogen | Horseradish peroxidase (Grade I). |
| Specificity | The antibody HP-03 reacts with horseradish peroxidase. It is suitable for PAP detection system. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Ammonium sulfate precipitation |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | The antibody HP-03 was successfully used for staining of formaldehyde- fixed, Triton X- permeabilized cells transfected with HRP gene. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | Phosphate buffered saline (PBS), pH 7.4 |
| Preservative | 15mM Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Ammonium sulfate precipitation |
Secondary Antibodies |
Isotype Controls |
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Ultrasensitive IHC Detection with HRP-Polymer Conjugates Signal amplification methods are widely used in immunohistochemistry (IHC) for detection of rare epitopes and low abundance antigens. While many of these techniques such as the avidin-biotin complex (ABC) method improve staining, they frequently re... Read full blog post. |
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5 Stars for the Lightning-Link HRP Antibody Labeling Kit Novus' Lightning-Link HRP Antibody Labeling Kit (cat# 701-0000) has received some glowing product reviews and customer feedback lately. One satisfied customer wrote, "Fast, efficient, stable direct HRP conjugation - best reagent we have found..." Anot... Read full blog post. |
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")