HPa3 Antibody (HIC3-2D12) - BSA Free

Immunohistochemistry-Paraffin: HPa3 Antibody (HIC3-2D12) [NBP3-18520] - Formalin Fixed Paraffin Embedded (FFPE) human pancreatic tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:50 dilution of Cat. No. MABS1998, Anti-HPa3, clone HIC3-2D12. Reactivity was detected using an Rabbit Anti-Mouse and DAB. Cytoplasmic/membranous staining in islets of human pancreas (Left image). membranous staining in intercalated ducts (Middle image), as well as intralobular ducts of human pancreas (Right image) tissues was observed.

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: HIC3-2D12
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 2 mg/ml

HPa3 Antibody (HIC3-2D12) - BSA Free Summary

• Immunogen: Human pancreatic islets enriched in non-beta endocine cells.
• Specificity: This HPa3 antibody does not label beta cells, labels delta cells dimly, and brightly labels alpha, gamma, and epsilon cells in human pancreatic islets.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry reported in scientific literature (PMID 27399229)
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50. Use reported in scientific literature (PMID 27399229)

Packaging, Storage & Formulations

• Storage: Store at 4°C.
• Buffer: PBS
• Preservative: 0.09% Sodium Azide
• Concentration: 2 mg/ml
• Purity: Ion exchange chromatography

Background

Stem cells offer a therapeutic promise for many diseases. In the case of diabetes, stem cells offer the promise that beta cells may be generated ex vivo for the possible transplantation and cure of Type I diabetes (Couri CE, et al.). For this to happen, stem cells will need to be isolated and expanded, they will need to be differentiated toward the beta cell lineage, and the differentiated cell progeny used for transplantation may need to be isolated from complex cell mixtures following differentiation. Appropriate markers targeting cell surface molecules on developing and mature pancreatic cells will be required for such research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HPa3 Antibody (NBP3-18520)(1)

Publication using NBP3-18520

• Dorrell C, Schug J, Canaday P et al. Human islets contain four distinct subtypes of beta cells Nat Commun 2016-07-11 [PMID: 27399229] (FLOW, Human)