HMW E.Coli Host Cell Proteins Antibody - BSA Free

Western Blot: HMW E.Coli Host Cell Proteins Antibody [NBP2-44221] - 2D Western Blot of anti-E.coli High Molecular Weight Host Cell Protein antibody. Load: 35 Ug HMW HCP.

ELISA: HMW E.Coli Host Cell Proteins Antibody [NBP2-44221] - ELISA of Rabbit anti-High Molecular Weight Host Cell Protein Antigen: BSA conjugates of HMW HCP. Coating amount: 1 Ug per well titrated 1:3 in 1XPBS.

• Reactivity: Ec
• Applications: WB, DB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

HMW E.Coli Host Cell Proteins Antibody - BSA Free Summary

• Description: This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative
  
  Store this antibody at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: HMW E.Coli Host Cell Proteins Antibody was prepared by repeated immunizations with a mixture high molecular weight (HMW) proteins from E.Coli (BL21).
• Isotype: Serum
• Clonality: Polyclonal
• Host: Rabbit
• Purity: Delipidation and Defibrination

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA 1:1000-1:2000
  • Western Blot 1:500-1:1000
• Application Notes: This product detects over 250 E.coli HMW proteins and has been tested in 2D, western blotting, and ELISA.

Reactivity Notes

This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is directed against E.coli Host Cell Proteins. Cross-reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Delipidation and Defibrination

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.