![Dot Blot: HIV-1 Protease Antibody (1696) - BSA Free [NB500-337] - Fig. 1. Dot Blot analysis of recombinant HIV-1 Protease. The total amount of recombinant HIV-protease spotted on the nitrocellulose membrane are indicated in left column. Lane 1: anti-HIV-1 Protease (1696); 0.2 ug/ml Lane 2: anti-HIV-1 Protease (1696); 1.0 ug/ml Lane 3: anti-HIV-1 Protease (1696); 2.0 ug/ml](http://images.novusbio.com/fullsize/HIV-1-Protease-Antibody-1696-BSA-Free-Dot-Blot-NB500-337-img0002.jpg "Dot Blot: HIV-1 Protease Antibody (1696) - BSA Free [NB500-337] - Fig. 1. Dot Blot analysis of recombinant HIV-1 Protease. The total amount of recombinant HIV-protease spotted on the nitrocellulose membrane are indicated in left column. Lane 1: anti-HIV-1 Protease (1696); 0.2 ug/ml Lane 2: anti-HIV-1 Protease (1696); 1.0 ug/ml Lane 3: anti-HIV-1 Protease (1696); 2.0 ug/ml")
| Reactivity | VSpecies Glossary |
| Applications | WB, ELISA |
| Clone | 1696 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 1.0 mg/ml |
| Description | Novus Biologicals Mouse HIV-1 Protease Antibody (1696) - BSA Free (NB500-337) is a monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | Bacterially expressed full-length HIV-1 Protease |
| Specificity | The antibody 1696 recognizes free N-terminus of mature HIV protease (HIV-1 and HIV-2), an enzyme that hydrolyzes polyproteins of HIV viruses into functional proteins. The antibody 1696 does not react with the precursor. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Functional Application: The antibody 1696 strongly inhibits the enzyme activity of HIV-1 and HIV-2 proteases. |
| Storage | Store at 4C. Do not freeze. |
| Buffer | Phosphate buffered saline (PBS), pH 7.4 |
| Preservative | 15mM Sodium Azide |
| Concentration | 1.0 mg/ml |
| Purity | Protein A purified |
Secondary Antibodies |
Isotype Controls |
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![SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](https://images.novusbio.com/images/Mouse-IgG1-Isotype-Control-MG1-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control (MG1) [NBP1-97005] - Lane 1: , Non-reduced. M: Opal Pre-stained Ladder. Lane 2: , Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")