HIV-1 Gag p24 Antibody (2230C) [Unconjugated]

Western blot shows recombinant HIV Gag p24. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Viral HIV-1 Gag p24 Monoclonal Antibody (Catalog # MAB9539) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for HIV-1 Gag p24 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: V
• Applications: WB
• Clone: 2230C
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

HIV-1 Gag p24 Antibody (2230C) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant viral HIV-1 Gag p24
  Pro149-Gly354
  Accession # Q77YG1
• Specificity: Detects viral HIV-1 Gag p24 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HIV-1 Gag p24 Antibody (2230C) [Unconjugated]

• CA
• Capsid Protein
• HIV1 Gag p24
• HIV-1 Gag p24
• Pr55(Gag)

Background

The gag gene of human immunodeficiency virus 1 (HIV-1) encodes a precursor protein known as Pr55Gag. The viral protease PR cleaves this precursor to generate p17, p24, p7, and p6 proteins which are required for virus particle assembly. p24 is a major viral core structural protein. Its measurement is commonly used as an indicator of HIV-1 infection and viral load.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HIV-1 Gag p24 Antibody (MAB9539)(1)

Publication using MAB9539

• Haldrup, J;Andersen, S;Labial, ARL;Wolff, JH;Frandsen, FP;Skov, TW;Rovsing, AB;Nielsen, I;Jakobsen, TS;Askou, AL;Thomsen, MK;Corydon, TJ;Thomsen, EA;Mikkelsen, JG; Engineered lentivirus-derived nanoparticles (LVNPs) for delivery of CRISPR/Cas ribonucleoprotein complexes supporting base editing, prime editing and in vivo gene modification Nucleic acids research 2023-09-08 [PMID: 37678882] (Western Blot, Human)

Bioinformatics

• Uniprot:
  • Q77YG1()