Hemagglutinin/HA Peptide Antibody (2908B) [Unconjugated]

Hemagglutinin was detected in immersion fixed Transfected CHO Chinese hamster ovary cells (positive) and absent in Wild type CHO Chinese hamster ovary cells (negative) using Rabbit Anti-Influenza A Virus H1N1 Hemagglutinin Monoclonal Antibody (Catalog # MAB11396) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western blot shows lysates of rvInflluenza A H1N1 Hemagglutinin. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Influenza A Virus H1N1 Hemagglutinin Monoclonal Antibody (Catalog # MAB11396) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Hemagglutinin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western lane view shows lysates of recombinant protein, loaded at 0.2 mg/ml. A specific band was detected for Hemagglutinin at approximately 111 kDa (as indicated) using 25 µg/mL of Rabbit Anti-Influenza A Virus H1N1 Hemagglutinin Monoclonal Antibody (MAB11396). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: V
• Applications: WB, ICC/IF
• Clone: 2908B
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Hemagglutinin/HA Peptide Antibody (2908B) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell, HEK293-derived influenza a virus H1N1 Hemagglutinin with a C-terminal 6-His tag
  Met1-Gln529
  Accession # YP_009118626
• Specificity: Detects Influenza A Virus H1N1 Hemagglutinin in direct ELISA.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 3-15 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Hemagglutinin/HA Peptide Antibody (2908B) [Unconjugated]

• HA
• Hemagglutinin

Background

Influenza hemagglutinin (HA) is a homotrimeric glycoprotein found on the surface of influenza viruses. HA is responsible for binding influenza virus to sialic acid on the surface of target cells. HA is also responsible for the fusion of the viral envelope with the late endosomal protein when it is exposed to low pH. H1N1 is a subtype of Hemagglutinin in influenza A which also includes Neurominidase protein on its surface. 

1. Russell, R.J. Kerry, P.S. Stevens, D.J. Steinhauer, D.A. Martin, S.R. Gamblin, S.H. Skehel, J.J. (November, 2008). "Structure of Invluenza Hemagllutinin in Complex with an Inhibitor of Membrane Fusion". Proceedings of the National Academy of Sciences of the United States of America. 105(46):17736-41.
2. Edinger, T.O. Pohl, O. Sertz, S. (February, 2014). "Entry of Influenza A. Virus: Host Factors and Antiviral Targets" (PDF). The Journal of General Virology. 95(Pt 2):263-277.
3. Horvath, P. Helenius, A. Yamauchi, Y. Banerjee, I. 12, July 2013. "High-Content Analysis of Sequential Events during the Early Phase of Influenza A Virus Infection". PLOS ONE. 8(7):e68450.
4. "Influenza Type A Viruses". Avian Influenza (Flu). CDC. 19, April 2017. Retrieved 27, August, 2018.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

Uniprot