Recombinant Human HDDC3 His Protein

SDS-Page: Recombinant Human HDDC3 Protein [NBP1-72506] - 15% SDS-PAGE (3ug)

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human HDDC3 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-140 of Human HDDC3
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSEAAQLLE AADFAARKHR QQRRKDPEGT PYINHPIGVA RILTHEAGIT DIVVLQAALL HDTVEDTDTT LDEVELHFGA QVRRLVEEVT DDKTLPKLER KRLQVEQAPH SSPGAKLVKL ADKLYNLRDL NRCTPEVKIQ

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: HDDC3
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 17.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15 M NaCl, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human HDDC3 His Protein

• (ppGpp)ase
• EC 3.1.7.2
• guanosine-3'-5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1
• HD domain containing 3
• HD domain-containing protein 3
• MESH1
• Metazoan SpoT homolog 1
• metazoan SpoT homolog-1
• MGC45386
• Penta-phosphate guanosine-3'-pyrophosphohydrolase

Background

HDDC3, also known as guanosine-3',5'-bis(diphosphate)-pyrophosphohydrolase MESH1, contains an active site for ppGpp hydrolysis and a conserved His-Asp-box motif for Mn(2+) binding. Consistent with these structural data, HDDC3 efficiently catalyzes hydrolysis of guanosine 3',5'-diphosphate (ppGpp) both in vitro and in vivo. HDDC3 also suppresses SpoT-deficient lethality and RelA-induced delayed cell growth in bacteria. Recombinant human HDDC3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HDDC3