HBsAg Antibody (S1-210)

Product Discontinued

HBsAg Antibody (S1-210) Summary

• Immunogen: Hepatitis B surface antigen.
• Localization: cytoplasm
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: S
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:40
• Application Notes: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS with 1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for HBsAg Antibody (S1-210)

• HBsAg
• HBV major surface antigen
• HBV surface antigen
• Hepatitis B Virus major surface antigen
• Hepatitis B Virus Surface Antigen
• Major surface antigen
• protein S

Background

Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr. Hepatitis B virus is a major causative agent of acute and chronic liver disease in humans. Hepatitis B surface antigen (HBsAg) is a protein component of the viral envelope, which is expressed predominantly in the cytoplasm of infected hepatocytes. Immunogen: Hepatitis B surface antigen.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HBsAg Antibody (NBP1-51726)(1)

Publication using NBP1-51726

• Chen X, Lai J, Pan Q et al. The delivery of HBcAg via Tat-PTD enhances specific immune response and inhibits Hepatitis B virus replication in transgenic mice. Vaccine 2010 May 21 [PMID: 20394723] (IHC, Mouse)

Bioinformatics

• Gene Symbol: S