HBsAg Antibody (HB12)


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Product Details

Reactivity V-ViSpecies Glossary
Applications S-ELISA

HBsAg Antibody (HB12) Summary

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant HBsAg of ayw subtype.
Virion membrane
Reacts with the following HbsAg subtypes : ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-, ayw3 (Fer).
Protein G purified
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  • Sandwich ELISA 1:100-1:2000
Application Notes
Recommended pairs for sandwich immunoassay: NB110-7392 - NB110-7391, NB110-7392 - NB100-73135.

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
PBS (pH 7.4)
0.09% Sodium Azide
Protein G purified

Alternate Names for HBsAg Antibody (HB12)

  • HBsAg
  • HBV major surface antigen
  • HBV surface antigen
  • Hepatitis B Virus major surface antigen
  • Hepatitis B Virus Surface Antigen
  • Major surface antigen
  • protein S


Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase. Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed. The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 405 NB110-7392AF405
Alexa Fluor 488 NB110-7392AF488
Alexa Fluor 647 NB110-7392AF647
Alexa Fluor 700 NB110-7392AF700
Biotin NB110-7392B
DyLight 350 NB110-7392UV
DyLight 405 NB110-7392V
DyLight 488 NB110-7392G
DyLight 550 NB110-7392R
DyLight 650 NB110-7392C
DyLight 680 NB110-7392FR
DyLight 755 NB110-7392IR
FITC NB110-7392F
HRP NB110-7392H

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Gene Symbol S