HBsAg Antibody (3E7)

Immunohistochemistry-Paraffin: HBsAg Antibody (3E7) [NBP1-22568] - Formalin fixed paraffin embedded infected liver stained with HBsAg antibody (NBP1-22568).

Immunohistochemistry-Paraffin: HBsAg Antibody (3E7) [NBP1-22568] - Human liver.

Immunohistochemistry-Paraffin: HBsAg Antibody (3E7) [NBP1-22568] - Staining in mouse liver, 1:50 antibody dilution, image from a verified customer review.

Product Discontinued

HBsAg Antibody (3E7) Summary

• Immunogen: Purified HBsAg subtype ayw3.
• Localization: Cytoplasmic
• Specificity: This reacts with the 'a' determinant present on the HBsAg subtypes ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4 and adr. It does not react with normal tissues.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: S
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:25-1:50
• Application Notes: Analyte Specific Reagent. This antibody stains the cytoplasm of antigen-positive liver cells infected with Type B viral hepatitis. IHC-P reported in a verified customer review. Use in Flow Cytometry reported in scientific literature (PMID:33125875).

Reactivity Notes

Reacts to Hepatitis B virus (HBV). Human reactivity reported in scientific literature (PMID:33125875).

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Tissue culture supernatant
• Preservative: 0.09% Sodium Azide
• Purity: Tissue culture supernatant

Alternate Names for HBsAg Antibody (3E7)

• HBsAg
• HBV major surface antigen
• HBV surface antigen
• Hepatitis B Virus major surface antigen
• Hepatitis B Virus Surface Antigen
• Major surface antigen
• protein S

Background

Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for HBsAg Antibody (NBP1-22568)(2)

Publications using NBP1-22568

• Gupta MK, Vethe H, Softic S et al. Danicopan: an oral complement factor D inhibitor for paroxysmal nocturnal hemoglobinuria Stem Cell Reports Oct 29 2020 [PMID: 33125875] (Flow, Human)
• Yeganeh B, Rezaei Moghadam A, Alizadeh J et al. Hepatitis B and C virus-induced hepatitis: Apoptosis, autophagy, and unfolded protein response. World J Gastroenterol 2015-12-21 [PMID: 26715805]

Review for HBsAg Antibody (NBP1-22568) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 06/12/2019

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: mouse liver
• Species: Mouse
• Lot: Lot P359

Comments

• Comments: Primary Antibody 1:50; detection Kit MOM Polymer-HRP.
  Weak positive staining compare to other HBsAg Clone 3E7 Antibosy (other Brand).

Bioinformatics

• Gene Symbol: S