H-2Dd Antibody (34-5-8S) [PE]

Flow Cytometry: H-2Dd Antibody (34-5-8S) [PE] [NBP1-28188] - Analysis of DBA/2 splenocytes.

• Reactivity: Mu
• Applications: Flow, IP, B/N
• Clone: 34-5-8S
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE
• Concentration: 0.1 mg/ml

H-2Dd Antibody (34-5-8S) [PE] Summary

• Immunogen: BDF1 mouse splenocytes
• Specificity: A conformational epitope on the alpha1 and alpha2 chains of mouse H-2Dd class I alloantigen
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • Flow Cytometry 0.2 ug/10^6 cells
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS and a stabilizing agent
• Preservative: Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for H-2Dd Antibody (34-5-8S) [PE]

• H2Dd
• MHC class I

Background

H-2Dd MAb 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpaha1 and alpha2 chains when complexed with beta2-microglobulin. (2) The antibody does not react with H-2Dd alpha chains synthesized in vitro. (3) Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. (4) Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. (2) MAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells. (5-9)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.