H-2Dd Antibody (34-5-8S) [PE]

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Flow Cytometry: H-2Dd Antibody (34-5-8S) [PE] [NBP1-28188] - Analysis of DBA/2 splenocytes.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications B/N, Flow, IP
Clone
34-5-8S
Clonality
Monoclonal
Host
Mouse
Conjugate
PE
Concentration
0.1 mg/ml

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H-2Dd Antibody (34-5-8S) [PE] Summary

Immunogen
BDF1 mouse splenocytes
Specificity
A conformational epitope on the alpha1 and alpha2 chains of mouse H-2Dd class I alloantigen
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Blocking/Neutralizing 50-200 molar excess
  • Flow Cytometry 0.2 ug/10^6 cells
  • Immunoprecipitation 1:10-1:500

Reactivity Notes

Mouse. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS and a stabilizing agent
Preservative
Sodium Azide
Concentration
0.1 mg/ml
Purity
Protein A or G purified

Notes

Protect conjugated forms from light.

Alternate Names for H-2Dd Antibody (34-5-8S) [PE]

  • H2Dd
  • MHC class I

Background

H-2Dd MAb 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpaha1 and alpha2 chains when complexed with beta2-microglobulin. (2) The antibody does not react with H-2Dd alpha chains synthesized in vitro. (3) Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. (4) Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. (2) MAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells. (5-9)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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