H-2Dd Antibody (34-5-8S) - Azide and BSA Free

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Flow Cytometry: H-2Dd Antibody (34-5-8S) [NBP1-28185] - Analysis using the Biotin conjugate of NBP1-28185. Staining of DBA/2 splenocytes.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow
Clone
34-5-8S
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
0.5 mg/ml

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Catalog# & Conjugate Size Price

H-2Dd Antibody (34-5-8S) - Azide and BSA Free Summary

Immunogen
BDF1 mouse splenocytes
Specificity
A conformational epitope on the alpha1 and alpha2 chains of mouse H-2Dd class I alloantigen
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Purity
Protein A or G purified
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Applications/Dilutions

Dilutions
  • Flow Cytometry 1:10-1:1000

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
0.1M BBS (pH 8.2)
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
Protein A or G purified

Alternate Names for H-2Dd Antibody (34-5-8S) - Azide and BSA Free

  • H2Dd
  • MHC class I

Background

MAb 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpaha1 and alpha2 chains when complexed with beta2-microglobulin. (2) The antibody does not react with H-2Dd alpha chains synthesized in vitro. (3) Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. (4) Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. (2) MAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells. (5-9)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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