GTF2H2C Antibody (PCRP-GTF2H2C-2C9)

Immunocytochemistry/Immunofluorescence: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154] - Immunofluorescence analysis of PFA-fixed HeLa cells. GTF2H2C Mouse Monoclonal Antibody (PCRP-GTF2H2C-2C9) followed by goat anti- Mouse IgG-CF488 (green); phalloidin (red).

Immunohistochemistry-Paraffin: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with GTF2H2C Mouse Monoclonal Antibody (PCRP-GTF2H2C-2C9).

Flow Cytometry: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154] - Flow Cytometric Analysis of PFA-fixed HeLa cells. GTF2H2C Mouse Monoclonal Antibody (PCRP-GTF2H2C-2C9) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).

Immunohistochemistry-Paraffin: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154] - Formalin-fixed, paraffin-embedded human breast carcinoma stained with GTF2H2C Mouse Monoclonal Antibody (PCRP-GTF2H2C-2C9).

Protein Array: GTF2H2C Antibody (PCRP-GTF2H2C-2C9) [NBP3-07154] - Analysis of Protein Array containing more than 19,000 full-length human proteins using GTF2H2C Mouse Monoclonal Antibody (PCRP-GTF2H2C-2C9).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, MA
• Clone: PCRP-GTF2H2C-2C9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

GTF2H2C Antibody (PCRP-GTF2H2C-2C9) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08896)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human GTF2H2C protein (Uniprot: Q6P1K8)
• Localization: Nucleus. Nuclear speckles.
• Isotype: IgG2c
• Clonality: Monoclonal
• Host: Mouse
• Gene: GTF2H2C
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1-2 ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Protein Array
  • Western Blot 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react in Mouse and Rat.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for GTF2H2C Antibody (PCRP-GTF2H2C-2C9)

• General transcription factor IIH polypeptide 2-like protein
• general transcription factor IIH subunit 2-like protein
• general transcription factor IIH, polypeptide 2C
• GTF2H2C_2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GTF2H2C
• Uniprot:
  • Q6P1K8(Human)