Growth Cone Antibody (2G13)

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Immunohistochemistry-Paraffin: Growth Cone Antibody (2G13) [NB600-785] - Staining of growth cones of primary olfactory neurons within the olfactory bulb in the developing rat brain using NB 600-785.

Product Details

Summary
Reactivity Mu, Rt, ChSpecies Glossary
Applications IHC, IHC-Fr, IHC-P
Clone
2G13
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Growth Cone Antibody (2G13) Summary

Immunogen
Embryonic chick tectal membranes
Specificity
This is useful as a general reagent for the detection of axonal growth cones in tissue sections and potentially a key marker in neuronal development research.
Isotype
IgM
Clonality
Monoclonal
Host
Mouse
Purity
Tissue culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody can be used in Immunohistochemistry. *Optimal dilutionsshould be determined by the end user. This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. This antibody does not work in Flow Cytometry.
Publications
Read Publications using
NB600-785 in the following applications:

  • IHC
    1 publication

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tissue Culture Supernatant
Preservative
0.09% Sodium Azide
Purity
Tissue culture supernatant

Background

This antibody recognises an intracellular protein 2G13p which is restricted to growth cones in developing brain and embryonic neurons in culture. The protein 2G13p also appears to be associated with filamentous actin in the growth cones suggesting a role for 2G13p in growth cone motility.This antibody is suitable for use in immunohistological studies of both frozen and paraffin-embedded sections, staining growth cones, including their filopodia and lamellipodies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Growth Cone Antibody (NB600-785)(5)

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Secondary Antibodies

 

Isotype Controls

Additional Growth Cone Products

Array NB600-785

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