Granzyme G Antibody [Biotin]

• Reactivity: Mu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

Granzyme G Antibody [Biotin] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Granzyme G
  Ile21-Leu248
  Accession # P13366
• Specificity: Detects mouse Granzyme G in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant mouse Granzyme D is observed and less than 1% cross‑reactivity with recombinant human Granzyme H is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: GZMG
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Granzyme G Antibody [Biotin]

• Ctla7
• Ctla-7
• Granzyme G
• GrzG
• GZMG

Background

Granzyme G is a member of the Granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1). Together with Granzymes D, E and F, it is regulated through pregnancy and by IL-2 and IL-15 in granulated metrial gland cells (2). Human or rat counterpart of mouse Granzyme G has not been found. Like other Granzymes, mouse Granzyme G is not secreted as a zymogen but stored as a fully processed and activated enzyme in the cytoplasmic granules of CTL (3). It is synthesized as a 248 amino acid precursor with a 18 amino acid signal peptide and a 2 amino acid propeptide (3, 4). The mature protein (residues 21‑248) is expressed and purified.

1. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
2. Allen, M.P. and M. Nilsen-Hamilton (1998) J. Immunol. 161:2772.
3. Jenne, D.E. et al. (1989) Biochemistry 28:7953.
4. Kwon, B.S. et al. (1988) J. Exp. Med. 168:1839.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GZMG
• Uniprot:
  • P13366()